Citation: Ru-Yi Chen, Teng Zhao, Jing-Jing Guo, Feng Zhu, Na-Na Zhang, Xiao-Feng Li, Hai-Tao Liu, Fei Wang, Yong-Qiang Deng, Cheng-Feng Qin. The infection kinetics and transmission potential of two Guaico Culex viruses in Culex quinquefasciatus mosquitoes .VIROLOGICA SINICA, 2024, 39(2) : 228-234.  http://dx.doi.org/10.1016/j.virs.2024.03.002

The infection kinetics and transmission potential of two Guaico Culex viruses in Culex quinquefasciatus mosquitoes

  • Guaico Culex virus (GCXV) is a newly identified segmented Jingmenvirus from Culex spp. mosquitoes in Central and South America. The genome of GCXV is composed of four or five single-stranded positive RNA segments. However, the infection kinetics and transmission capability of GCXV in mosquitoes remain unknown. In this study, we used reverse genetics to rescue two GCXVs (4S and 5S) that contained four and five RNA segments, respectively, in C6/36 cells. Further in vitro characterization revealed that the two GCXVs exhibited comparable replication kinetics, protein expression and viral titers. Importantly, GCXV RNAs were detected in the bodies, salivary glands, midguts and ovaries of Culex quinquefasciatus at 4–10 days after oral infection. In addition, two GCXVs can colonize Cx. quinquefasciatus eggs, resulting in positive rates of 15%–35% for the second gonotrophic cycle. In conclusion, our results demonstrated that GCXVs with four or five RNA segments can be detected in Cx. quinquefasciatus eggs during the first and second gonotrophic cycles after oral infection.

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    The infection kinetics and transmission potential of two Guaico Culex viruses in Culex quinquefasciatus mosquitoes

      Corresponding author: Fei Wang, wangfei179@163.com
      Corresponding author: Yong-Qiang Deng, dengyq1977@126.com
      Corresponding author: Cheng-Feng Qin, qincf@bmi.ac.cn
    • a. College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, 330045, China;
    • b. State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, AMMS, Beijing, 100071, China;
    • c. School of Life Sciences, Southwest Forestry University, Kunming, 650224, China

    Abstract: Guaico Culex virus (GCXV) is a newly identified segmented Jingmenvirus from Culex spp. mosquitoes in Central and South America. The genome of GCXV is composed of four or five single-stranded positive RNA segments. However, the infection kinetics and transmission capability of GCXV in mosquitoes remain unknown. In this study, we used reverse genetics to rescue two GCXVs (4S and 5S) that contained four and five RNA segments, respectively, in C6/36 cells. Further in vitro characterization revealed that the two GCXVs exhibited comparable replication kinetics, protein expression and viral titers. Importantly, GCXV RNAs were detected in the bodies, salivary glands, midguts and ovaries of Culex quinquefasciatus at 4–10 days after oral infection. In addition, two GCXVs can colonize Cx. quinquefasciatus eggs, resulting in positive rates of 15%–35% for the second gonotrophic cycle. In conclusion, our results demonstrated that GCXVs with four or five RNA segments can be detected in Cx. quinquefasciatus eggs during the first and second gonotrophic cycles after oral infection.

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