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Volume 22 Issue 5
October 2007
    Citation: Guang-qing LIU, Fei WANG, Zheng NI, Tao YUN, Bin YU, Jiong-gang HUANG, Jian-Ping CHEN. Complete Genomic Sequence of a Chinese Isolate of Duck Hepatitis Virus* .VIROLOGICA SINICA, 2007, 22(5) : 353-359.
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    Complete Genomic Sequence of a Chinese Isolate of Duck Hepatitis Virus*

    • 1.

      Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

    • 2.

      College of Animal Science and Technology, Anhui Agriculture University, Hefei 230036, China

    • Corresponding author: Guang-qing LIU, liuguangqing@hotmail.com
      Jian-Ping CHEN, liuguangqing@hotmail.com
    • Received Date: 27 February 2007
      Accepted Date: 17 August 2007
      Available online: 01 October 2007

      Fund Project: National Natural Science Foundation of China 30670074

    • The complete genomic sequence of Duck hepatitis virus 1 (DHV-1) ZJ-V isolate was sequenced and determined to be 7 691 nucleotides (nt) in length with a 5′-terminal un-translated region (UTR) of 626 nt and a 3′-terminal UTR of 315 nt (not including the poly(A) tail). One large open reading frame (ORF) was found within the genome (nt 627 to 7 373) coding for a polypeptide of 2 249 amino acids. Our data also showed that the poly (A) tail of DHV-1 has at least 22 A's. Sequence comparison revealed significant homology (from 91.9% to 95.7%) between the protein sequences of the ZJ-V isolate and those of 21 reference isolates. Although DHV-1 has been classified as an unassigned virus in the Picornaviridae family, its genome showed some unique characteristics. DHV-1 contains 3 copies of the 2A gene and only 1 copy of the 3B gene, and its 3′-NCR is longer than those of other picornaviruses. Phylogenetic analysis to do sequence homology based on the VP1 protein sequences showed that the ZJ-V isolate shares high sequence homology with the reported DHV-1 isolates (from 92.9% to 99.2%), indicating that DHV-1 is genetically stable.
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      2. 2. Forss S, Strebel K, Beck E, et al.1984.Nucleotide sequence and genome organization of foot-and-mouth disease virus.Nucleic Acids Res, 12:6587-6601.
          doi: 10.1093/nar/12.16.6587

      3. Gromeier M, Wimmer E, Gorbalenya A E. 1999. Genetics, pathogenesis and evolution of picornaviruses. In: Origin and evolution of viruses (Domingo E, Webster R, Holland J, Eds). Academic Press. p287-343.

      4. 4. Haider S A, Calnek B W.1979.In vitro isolation, propagation, and characterization of duck hepatitis virus type Ⅲ.Avian Dis, 23:715-729.
          doi: 10.2307/1589748

      5. 5. Kanno T, Yamakawa M, Yoshid K, et al.2002.The complete nucleotide sequence of the PanAsia Isolate of foot-and-mouth disease virus isolated in Japan.Virus Genes, 25:119-125.
          doi: 10.1023/A:1020103700108

      6. 6. Kim M C, Kwon Y K, Joh S J, et al.2006.Molecular analysis of duck hepatitis virus type 1 reveals a novel lineage close to the genus Parechovirus in the family Picornaviridae.J Gen Virol, 87:3307-3316.
          doi: 10.1099/vir.0.81804-0

      7. 7. Levine P P, Fabricant J.1950.A hitherto-undescribed virus disease of ducks in North America.Cornell Vet, 40:71-86.

      8. McNulty M S. 2001. Picornaviridae. In: Poultry Diseases (Jordan F, Pattison M, Alexander 584 D, et al Eds). 5th ed. London: W. B. Saunders. 305-318

      9. 9. Robertson B H, Grubman M J, Weddell G N, et al.1985. Nucleotide and amino acid sequence coding for poly-pepides of foot-and-mouth disease virus type A12.J Virol, 54:651-660.

      10. 10. Rohll J B, Moon D H, Evans D J, et al.1995.The 3' untranslated region of Picornavirus RNA: features required for efficient genome replication.J Virol, 69:7835-7844.

      11. 11. Toth T E.1969.Studies of an agent causing mortality among ducklings immune to duck virus hepatitis.Avian Dis, 13:834-846.
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      12. 12. Tseng C H, Knowles N J, Tsai H J. 2007. Molecular analysis of duck hepatitis virus type 1 indicates that it should be assigned to a new genus. Virus Res, 123: 190-203.
          doi: 10.1016/j.virusres.2006.09.007

      13. 13. Wildy P. 1997.Classification and nomenclature of viruses: first report of the International Committee on Taxonomy of Viruses. Monogr Virol, 5: 1-118.

      14. Woolcock P R. 2003. Duck hepatitis. In: Diseases of poultry (Saif Y M, Barnes H J. Glisson J R, et al Eds). 11th ed. Ames: Iowa State Press. p343-354.

      15. 15. Wutz G, Auer H, Nowotny N, et al. 1996. Equine rhinovirus serotypes 1 and 2: relationship to each other and to aphthoviruses and cardioviruses. J Gen Virol, 77: 1719-1730.
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      16. 16. Zell R., Dauber M, Krumbholz A, et al.2001.Porcine teschoviruses comprise at least eleven distinct serotypes: molecular and evolutionary aspects.J Virol, 75:1620-163.
          doi: 10.1128/JVI.75.4.1620-1631.2001

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      Complete Genomic Sequence of a Chinese Isolate of Duck Hepatitis Virus*

        Corresponding author: Guang-qing LIU, liuguangqing@hotmail.com
        Corresponding author: Jian-Ping CHEN, liuguangqing@hotmail.com
      • 1. Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
      • 2. College of Animal Science and Technology, Anhui Agriculture University, Hefei 230036, China
      • Received Date: 27 February 2007
      • Accepted Date: 17 August 2007
      Fund Project:  National Natural Science Foundation of China 30670074

      Abstract: The complete genomic sequence of Duck hepatitis virus 1 (DHV-1) ZJ-V isolate was sequenced and determined to be 7 691 nucleotides (nt) in length with a 5′-terminal un-translated region (UTR) of 626 nt and a 3′-terminal UTR of 315 nt (not including the poly(A) tail). One large open reading frame (ORF) was found within the genome (nt 627 to 7 373) coding for a polypeptide of 2 249 amino acids. Our data also showed that the poly (A) tail of DHV-1 has at least 22 A's. Sequence comparison revealed significant homology (from 91.9% to 95.7%) between the protein sequences of the ZJ-V isolate and those of 21 reference isolates. Although DHV-1 has been classified as an unassigned virus in the Picornaviridae family, its genome showed some unique characteristics. DHV-1 contains 3 copies of the 2A gene and only 1 copy of the 3B gene, and its 3′-NCR is longer than those of other picornaviruses. Phylogenetic analysis to do sequence homology based on the VP1 protein sequences showed that the ZJ-V isolate shares high sequence homology with the reported DHV-1 isolates (from 92.9% to 99.2%), indicating that DHV-1 is genetically stable.

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