Citation: Xiu-lian SUN, Hui-yin PENG. Recent Advances in Biological Control of Pest Insects by Using Viruses in China* .VIROLOGICA SINICA, 2007, 22(2) : 158-162.

Recent Advances in Biological Control of Pest Insects by Using Viruses in China*

  • Corresponding author: Xiu-lian SUN,
  • Received Date: 24 November 2006
    Accepted Date: 01 March 2003
    Available online: 01 April 2007

    Fund Project:

  • Insect viruses are attractive as biological control agents and could be a feasible alternative to chemical insecticides in the management of insect infestations. This review describes recent advances in the development of wild-type and genetically modified viruses as insecticides. A new strategy of application of insect viruses in China is reviewed. Also, the assessment of biosafety of genetically modified Helicoverpa armigera Nucleopolyhedovirus (HearNPV) is emphasized as a case-study.

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    1. Chen Q, Li G H, Pang Y, et al.. 2000. Research developments of Spodoptera exigua Nucleopolyhedrovirus. Virologica Sinica. (Special issue): 234-235. (in Chinese)

    2. Dong LQ, Zhang K Q, Qiu B S. 2003. Evaluation of the feasibility of using lactose as the inducer in producing the viral enhancing factor from Helicoverpa armigera granulovirus in Escherichia coli BL21DE3. Microbiology, 30: 5-9.

    3. Hu R, Meng X L, Xu J P. 2001. Expression of 2.6 kb enhancin gene fragment from Helicoverpa armigera granulovirus. Virologica Sinica, 16: 364-368. (in Chinese)

    4. Liu X G, Yang G, Qiu B S. 2000. Expression of two truncated enhancin gene from Helicoverpa armigera granulovirus in E. coli and its preliminary bioassay. Acta Microbiol Sinica, 41: 168-172. (in Chinese)

    5. Liu Y P, Wang F H, Su Z J, et al. 2005. Effect of juvenile hormone analogues on multiplication of Spod optera litura nucleopolyhedrovirus. Acta Entomol Sinica, 48: 866-870. (in Chinese)

    6. Ou Y, Meng X L, Xu J P. 2000. Expression of 2.1kb enhancin gene fragment from Helicoverpa armigera granulovirus in Escherichia coli. Chinese J Biotechnol, 16: 595-598. (in Chinese)

    7. Peng H Y, Chen X W, Jiang Y, et al. 1998. Controlling Dendrolimus punctatus with Trichogramma dendrolimi carrying cytoplasmic polyhedrosis virus. Chinese J Biol Control, 14: 111-114. (in Chinese)

    8. Su Z, Chen Q, Li G H, Pang Y. 2001. Control of Spodoptera litura with mixture of SlNPV and its synergist. Chinese J Biol Control, 17: 23-25. (in Chinese)

    9. Sun X C, Cheng G Y, Zhou M Z, et al.. 2004, Laboratory and field analysis on the transferring of AaIT gene of recombinant Helicoverpa armigera Nucleopolyhedrovirus (HaSNPV-AaIT) to its surrounding organisms, Virologica Sinica, 20: 420-423. (in Chinese)

    10. Sun X L, Sun X C, Bai B K, et al. 2005. Production of polyhedral inclusion bodies from Helicoverpa armigera larvae infected with wild-type and recombinant HaSNPV.Biocont Sci Technol, 15: 353-366.
        doi: 10.1080/09583150400016944

    11. Sun X L, Sun X C, van der Werf W, et al.. 2004. Field inactivation of wild-type and genetically modified Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus in cotton. Biocont Sci Technol, 14: 185-192.
        doi: 10.1080/09583150310001655684

    12. Sun X L, van der Werf W, Felix F J J A, et al. 2006.Modelling biological control with wild-type and genetically modified baculoviruses in the Helicoverpa armigera-cotton system. Ecological Modelling, 198:387-398.
        doi: 10.1016/j.ecolmodel.2006.05.011

    13. Sun X L, Wang H L, Sun X C, et al. 2004. Biological activity and field efficacy of a genetically modified Helicoverpa armigera SNPV expressing an insect-selective toxin from a chimeric promote. Biol Control, 29: 124-137.
        doi: 10.1016/S1049-9644(03)00117-8

    14. Wang E W, Xu J P, Lu W, et al. 2005. Expression and functional analysis of a recombinant scorpion neurototoxin AaIT. J Wuhan Univ (Nat. Sci. Ed.), 51: 727-732. (in Chinese)

    15. Xu H G, Peng H Y, Liu J X, et al. 2002. Enhancement to Helicoverpa armigera Nucleopolyhedrovirus by chitinase of WHS3 strain. Virologica Sinica, 17: 239-242. (in Chinese)

    16. Yuan Z M, Meng X L, Liu S S. 2004. Wide-spectrum synergy of recombinant enhancin from insect baculovirus expressed in Escherichia coli. Chinese J Biol Control, 20: 31-33. (in Chinese)

    17. Zhang G Y, Sun X L, Zhang Z X, et al. 1995.Production and effectiveness of the new formulation of Helicoverpa virus pesticide-emulsifiable suspension. Virologica Sinica, 10: 242-247. (in Chinese)

    18. Zhang Z X, Zhang H Y, Niu G D, et al. 2003. Efficiency and safety evaluation of HaNPV suspension formulation containing Chlorfluazuron. Virologica Sinica, 18: 571-575. (in Chinese)

    19. Zhou M Z, Sun X L, Sun X C, et al.. 2005. Horizontal and vertical transmission of wild-type and recombinant Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus. J Invert Pathol, 89: 165-175.
        doi: 10.1016/j.jip.2005.03.005

    20. Zhu J Q, Lei C L, Peng H Y, et al. 2002. Studies on the effect of Trichogramma dendrolimi carrying NPV to suppress Helicoverpa armigera. Natural Enemies of Insects, 24: 20-25. (in Chinese)

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    Recent Advances in Biological Control of Pest Insects by Using Viruses in China*

      Corresponding author: Xiu-lian SUN,
    • State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
    Fund Project: 

    Abstract: Insect viruses are attractive as biological control agents and could be a feasible alternative to chemical insecticides in the management of insect infestations. This review describes recent advances in the development of wild-type and genetically modified viruses as insecticides. A new strategy of application of insect viruses in China is reviewed. Also, the assessment of biosafety of genetically modified Helicoverpa armigera Nucleopolyhedovirus (HearNPV) is emphasized as a case-study.

    • Viruses from several insect families can cause epizootics and may play an important role in controlling insect populations in nature. Thus, they are attractive biological control agents and could be a feasible alternative to chemical insecticides. A number of insect viruses have been registered as bioinsecticides to control pests in crops, forests, and pas tures around the world. In this paper, recent advances in the development of wild-type viruses as insecticides, the control efficacy and biosafety assessment of a genetically modified baculoviruses and a new strategy of application of insect viruses in China are reviewed.

    • To date, eight nucleopolyhedroviruses (NPVs), three granuloviruses (GVs) and one cypovirus (CPV) have been registered as commercial insecticides in China (Table 1). HearNPV is the first commercial insecticide authorized by the Ministry of Agriculture of the P.R. China (17). This product is the most produced virus insecticides in China estimated at about 1600 tons of formulated HearNPV in 2005 (Investment and consulting report on biological agrochemicals, 2006-2007). Spodoptera litura NPV and S. exigua NPV have also been applied at a large scale to control pests on vegetables (1, 8).

      Table 1.  Viruses authorized as commercial insecticide in China

      Recently, efforts to improve the control efficacy of viral insecticides by selecting enhancers have been effective. For example, combination of a chitin synthase inhibitor, chlorfluazuron, with HearNPV formulationat a concentration of 4.2 ppm reduced the median lethal time in 3rd instar H. armigera larvae from 4.90 days to 2.24 days (18). Chitinase, expressed by Serratia marcesscens, was also used to enhance the infectivity of HearNPV (15). An enhancing protein and its truncated fragment from a granulovirus have been expressed and used as synergists of HearNPV and AcMNPV in pest control (2, 3, 4, 6, 16).

      Techniques have also been developed to increase the yield of viruses in host larvae. For example, incorporation of a juvenile hormone analogue, methoprene, with S. litura NPV might increase virus yield by 227% (5). Similarly, the juvenile hormone analogue ZR515 had been used to increase the yield of HearNPV (Chinese paten: ZL98121687.0).

    • A major limitation to wide use of baculoviruses for insect control is their slow speed of action. To enhance the efficacy of HearNPV, it was genetically modified either by deletion of the ecdysteroid UDP-glucosyltransferase (egt) gene from the genome (the recombinant HearNPV-Δegt) or by insertion of an insectspecific scorpion toxin (AaIT) gene at the egt gene locus (the recombinant HearNPV-AaIT) (13). To develop the recombinant HearNPVs as a commercial insecticide, the efficacy and biosafety of the recombinant viruses have been investigated.

    • Application of HearNPV-AaIT on cotton plantations protected the fruit from damage by the bollworm better than HearNPV or HearNPV-Δegt over entire cotton seasons in 2000 and 2001. Yield of cotton lint from HearNPV-AaIT-treated plants was about 22% higher than from plantations treated with wild-type virus alone (13). At an optimal regime to produce the recombinant HearNPV in vivo, the yield of Hear NPV-AaIT was about 44% of the wild-type virus (10). When all conditions are the same, the cost to spray of formulated HearNPV-AaIT was about 50% higher than that of wild-type virus. By all accounts, the increased value of cotton lint after sprays of HearNPV-AaIT formulation is much higher than sprays of the wildtype virus (Table 2). Thus the potential for comercialization of the recombinant HearNPV is promising.

      Table 2.  Comparison of economic effects of application of wild-type and recombinant HearNPVs in cotton

    • To assess the risk of releasing recombinant HearNPV into the environment, the effects on nontarget species, possibility of AaIT gene flow to other organisms, and their environment fitness were studied.

      Effects on non-target species: The median lethal dose of HearNPV-AaIT was above 2 000 mg/kg against female and male rats administered by either intra dermal or per os. This recombinant was also found to be non toxic for bobwhite quail, zebra fish, silkworm and bees. It is not an allergen to a guinea pig skin. Furthermore, it there was no pathological response when rats were inoculated with the recombinant virus.

      In experiments of multiple application of HearNPV-AaIT on cotton in 2000, 2001 and 2002, biodiversity and the dynamics of the natural enemies were also monitored under field conditions. No significant difference in biodiversity and in the number of natural enemies was observed when plots were treated with either the recombinant or the wild-type virus (Sun et al., unpublished data). Furthermore, the survival time and fecundity of ladybeetles did not differ significantly when they were fed H. armigera larvae infected with either HearNPV-AaIT or wildtype virus (Sun et al., unpublished data). The toxin AaIT expressed by yeast was biologically active against Spodoptera exigua and Argyrogramma agnata larvae when injected into the haemocoel. Whereas, it was not toxic to the two insect species when the toxin was administered per os (14). These data indicate that AaIT was active when it was injected into or expressed in the neurosystem of the insects and it was not active when it was given per os.

      Possibility of transferring of AaIT gene from recombinant virus to its surrounding organisms: PCR and dot-blot hybridization were used to test the possiblity AaIT gene flow from HearNPV-AaIT to other organisms after field release. AsIT sequences were not detected in the genome of a pathogen of cotton, Verticillium dahliae Lleb., which was cocultivated with HearNPV-AaIT DNA or virions for up to 90 days. Similarly, AsIT sequences were not detected in the genomic DNA of ladybeetles collected from fields where the recombinant virus was applied several times (9). It is concluded that possibility of the AaIT gene of the recombinant HearNPV transferred to surrounding organisms was very low.

      Fitness of the recombinant HearNPV in the environment: Virus yield per larva, inoculated with HearNPV-AaIT in the 1st, 2nd, 3rd, 4th or 5th larval stage, was 23%, 32%, 41%, 44% and 47% of the yield of HearNPV-wt, respectively (14). These data indicate that production of recombinant virus in host larvae was lower than that of wild-type virus.

      In the field, there was no significant difference among the inactivation rates of the two recombinant HearNPVs and the wild-type virus on cotton plants (11). Furthermore, the persistence of recombinant viruses in soil was not significantly different from that of wild-type virus (12).

      In the field, HearNPV-AaIT exhibited a significantly lower rate of transmission than the wild-type virus. The vertical transmission of HearNPV-AaIT from infected females to offspring was also significantly lower than that of wild-type HearNPV (19).

      Based upon the results mentioned above, there is no evidence that the recombinant baculovirus provides an increased hazard to non target organisms including humans or has a deleterious effect on environmental health in comparison to wild-type viruses. In order to commercialize the genetically modified HearNPV containing AaIT gene, a data package on the safety the virus has been submitted to the Ministry of Agriculture, P.R. China.

    • A new method for delivering viruses to target insects by using Trichogramma spp. as vector has been developed in China (7). By taking into account the biology and the ecology of T. dendrolimi, a special box was designed for releasing the wasps and accompanying viruses. When T. dendrolimi was released with Dendrolimus punctatus cypovirus to control the masson pine moth (D. punctatus), it was observed that 20%-40% of eggs had been parasitized and 40%-47% of D. punctatus larvae had been infected with the virus (7). In the case of release T. dendrolimi combined with HearNPV to control the cotton bollworm, it was observed that 33%-37% of eggs had been parasitized and 61%-70% of H. armigera larvae had been infected (20).

      This technique has also been used for control of H. armigera, S. litura, Plutella xylostella, Ectropis oblique, Pieris rapae, etc. on more than 20 000 hectares since 1991 in China.

    • The abuse of chemical insecticides has led to pest resistance, resurgence and environmental problems in many developing countries. Society now demands safer pesticides and less-toxic chemical residues in food and in water. Effective management of pests requires diverse and flexible tools. Among classical biocontrol agents, insect viruses are attractive tools that appear supplement or and, in many cases, replace synthetic chemical pesticides in IPM schemes. Despite their high potential and documented cases of success, the market for viral insecticides is still limited in China. The problem is mainly derived from their relative slow speed of action, relatively low virulence for the older instars and a narrow host range. However, many of these perceived disadvantages can be addressed by modern molecular technologies or in combination with other biocontrol strategies. Genetically modified baculoviruses have already been eluded to and appear to be effective in insect pest control. Furthermore, they can be produced in large amounts by village industries, indicating that they have the potential to deliver sustainable pest control, especially in developing countries.

    Table (2) Reference (20) Relative (20)



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