Citation: CHEN Gang, ZHOU Guang-He, CHEN Jian-Bo. Purification and Partial characterization of Maize Dwarf Mosaic Virus .VIROLOGICA SINICA, 1992, 7(1) : 93.

Purification and Partial characterization of Maize Dwarf Mosaic Virus

  • Available online: 05 March 1992
  • Purified virus preparation of Maize Dwarf Mosaic Virus (MDMV) withhigh purity and infectivity was obtained by an improved procedure of puri-fication. The yield of purified virus was 0.7--1.2mg/100g infected tissue. Thevirus preparation had a typical ultraviolet absorption spectra of a filamentousvirus containing about 6% nucleic acid, A280/A260 was 0.63--0.80, Amax/Amin was 1.15. The particle size of purified virus was 720--750×14nm and themolecular weight of its coat protein was 36kd by SDS-PAGE. Antiserum...

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    Purification and Partial characterization of Maize Dwarf Mosaic Virus

    • 1. Institute of plant protection
    • 2. CAAS

    Abstract: Purified virus preparation of Maize Dwarf Mosaic Virus (MDMV) withhigh purity and infectivity was obtained by an improved procedure of puri-fication. The yield of purified virus was 0.7--1.2mg/100g infected tissue. Thevirus preparation had a typical ultraviolet absorption spectra of a filamentousvirus containing about 6% nucleic acid, A280/A260 was 0.63--0.80, Amax/Amin was 1.15. The particle size of purified virus was 720--750×14nm and themolecular weight of its coat protein was 36kd by SDS-PAGE. Antiserum...

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