A Single--Stranded Virus Isolated from Shiitake Mushroom Lentinus edodes (Berk.) Sing.
Abstract: A isometric virus-like particles(VLPs), which are approximately 34nm indiameter containing ss-RNA, have been purified from abnormal mycelial ofshiitake mushroom Leutinus edodes (Berk.) Sing. The SDS-polyacrylamide gelelectrophoresis demonstrated that the virions contain a single capsid polypeptideband with molecular weight about 22000 dalton. The nucleic acid extractedfrom purified preparations of 34nm VLPs showed only one band with mole-cular weight approximately 2.3810~6 dalton on the 1.5% agarose gel. T...