Rapid Detection of HBV-DNA by Using Biotinylated Oligonucleotide Probe
Abstract: A rapid method for detection of HBV-DNA was developed. This assay was based on the hybridization of biotinylated oligonucleotide probe to target DNA immobolized on nitrocellulose membrane which was then incubated with avidin-alkaline phosphatase conjugator and measured with chromogenic substrates. The practicality of the method was investigated. It was found that the method was fast (4hr),sensitive(1-10pg)and expedient for laboratory and clinical detection of HBV-DNA.