The concentration and purification of rabies virus suspension using ultrafiltration and column chro-matography

The active Rabies virus suspension was cultured in microcarrier culture of five litres bioreac-tor.After virus suspension was havested from microcarrier culture,the prdcess comprised clarifi-cation by centrifugation and filtration,concentration by ultrafiltration,purification by gel filtra-tion.The virus was inactivated with 1:4000β-propiolactone at 4℃for 24 hours.The results indicated that infective titer of the rabies virus was not declinged clearly,thatresidual calf serum protein,residual DNA content and antigenic values(NIH test)in all purifiedvirus samples were conformed to the WHO standards.The research described in this paper indicated that isolating,purifying method of active ra-bies virus was effective, It provided some basic information which could be useful in further forthe largescale production of inactivated rabies vaccines.