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Volume 11 Issue 3
June 1996
    Citation: CHEN Shang-Wu, CHEN Rui-Jun, HUANG Di, SHU Zhen-Yu, MA Jian-Quan. Amplification of the Intact Gene Coding Epstein-Barr Virus-Specific tIIym idine Kinase .VIROLOGICA SINICA, 1996, 11(3) : 287.
    shu

    Amplification of the Intact Gene Coding Epstein-Barr Virus-Specific tIIym idine Kinase

    • 1.

      Department of Biochemistry．Sun-Yet University of Medical Sciences，Guangzhou 510089Cancer Research InStitute，Sun-sen University of Medical＆iences．Guangzhou 510060

    • Available online: 05 September 1996
    • Based o1"1 Epstein—Barr virus(EBV)DNA (B95—8)genome sequenc3e data and the strut—ture BXLFlopen readingframe codingforEBV ~hymidinekinase(TK)，a pairofPCR pr／mers was designed and svnthesized Template DNA was extracted rapidly from B95—8 calIs harboring EBV bv using guanidinium isothioeyanate(GuSCN)and silica∞urse(cs)．A 1843bp A frag—ment containing intact EBV TK gene was amplified by polymerase chain reaction．The PCR prod—uct was verified by cleaving with NcoI
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      Amplification of the Intact Gene Coding Epstein-Barr Virus-Specific tIIym idine Kinase

      • 1. Department of Biochemistry．Sun-Yet University of Medical Sciences，Guangzhou 510089Cancer Research InStitute，Sun-sen University of Medical＆iences．Guangzhou 510060

      Abstract: Based o1"1 Epstein—Barr virus(EBV)DNA (B95—8)genome sequenc3e data and the strut—ture BXLFlopen readingframe codingforEBV ~hymidinekinase(TK)，a pairofPCR pr／mers was designed and svnthesized Template DNA was extracted rapidly from B95—8 calIs harboring EBV bv using guanidinium isothioeyanate(GuSCN)and silica∞urse(cs)．A 1843bp A frag—ment containing intact EBV TK gene was amplified by polymerase chain reaction．The PCR prod—uct was verified by cleaving with NcoI

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