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Volume 13 Issue 2
April 1998
    Citation: XU Dong-Gang, DAI Hao-Yang, NIU Jian-Zhang, HAN Feng-Lian, GAO Xin. Cloning NS3 gene of Hepatitis G Virus from Chinese Patients of Post·Transfusion Hepatitis and Its Sequence Analysis .VIROLOGICA SINICA, 1998, 13(2) : 166.
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    Cloning NS3 gene of Hepatitis G Virus from Chinese Patients of Post·Transfusion Hepatitis and Its Sequence Analysis

    • 1.

      1 Institute of Basic Medical Siences．Academy of Military Medical Sicences，Beijing 1008502 Health and Anti—epidemic Station for Hehei Province．Baoding 071000302 Hospital of PLA，Beijing 100039

    • Available online: 05 June 1998
    • Epitope of HGV NS3 protein was analyzed by Goldkey Program,and according to predicted results,primers of NS3 gene were synthesized.HGV RNA was extracted from patients of post transfusion hepatitis,cDNA fragment of NS3 was amplified by RT PCR.Target gene was cloned into pGEM T vector,and sequence analysis showed that the fragment of NS3 is 591bp,G+C is 58.37%.The homology of nucleotide and reduced amino acid between the isolate and other strains from America and China is 84.26%-86.13%,94.42%-94.92%,respectively.The homology between Chinese strains is higher than that between American.
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      Cloning NS3 gene of Hepatitis G Virus from Chinese Patients of Post·Transfusion Hepatitis and Its Sequence Analysis

      • 1. 1 Institute of Basic Medical Siences．Academy of Military Medical Sicences，Beijing 1008502 Health and Anti—epidemic Station for Hehei Province．Baoding 071000302 Hospital of PLA，Beijing 100039

      Abstract: Epitope of HGV NS3 protein was analyzed by Goldkey Program,and according to predicted results,primers of NS3 gene were synthesized.HGV RNA was extracted from patients of post transfusion hepatitis,cDNA fragment of NS3 was amplified by RT PCR.Target gene was cloned into pGEM T vector,and sequence analysis showed that the fragment of NS3 is 591bp,G+C is 58.37%.The homology of nucleotide and reduced amino acid between the isolate and other strains from America and China is 84.26%-86.13%,94.42%-94.92%,respectively.The homology between Chinese strains is higher than that between American.

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