Citation: LENG Yan, CHEN Xiuzhu, DU Yong, MAO Chun-sheng, Du Guixin, WANG Haitao. Preliminary Study of Hepatitis C Viral Quasispecies in NS2 Region in Patients wiUl Hepatitis C Virus Infection .VIROLOGICA SINICA, 2001, 16(3) : 215-219.

Preliminary Study of Hepatitis C Viral Quasispecies in NS2 Region in Patients wiUl Hepatitis C Virus Infection

  • Available online: 05 September 2001
  • To explore the genetic variation of nonstmetural(NS)2 region and its gene structural characteristics in hepatitis C vims quasispecies,NS2 genes ofHCV from a HCV as)~nptomatie~[Tier and apatien twith ab. nomud ALT level tteile amphfied by RT-nested-PCR respectively and cloned into T Yector.5 positive clones form each of them were selected~ domly for sequencing.Eve~-clone WaS unique in nuclectide sequence and amino acid sequence.A 0Bt all the NS2 gene clones from the asymptomatic carrier had complete ORF ex。ept OIle done had a stop codon at 835aain HCV polypmtein.Butwhenit ean3~tothepatientwith abnorm al ALT level,3 clones of them had stop cadom.at 835aa and 1 clone had a stop ced on at 887aa in polypretein. 0nly 1 clone had complete ORF.Comparing this ORF with the s~ ence of the as)~lptomatic carrier,most of the~aino acid changes were confined to the N-termlnai.Simulating the so。ondarv strucRu~s of Ns2 proteins with intact openreadingframe demomUated thatthe structure ofthe HCV patientwith abnormalA levelwas similart0 the dominant structure ofthe asymptomatic carrier.It could beseenfrom thetwopatientswith HCV infection thatHepatitis C viral quasispeciesin NS2 r 0nhadan obvious diversity between HCv as)~lptoraatic p~riier and HCV patient with abnomaal A leve1.The diversity suggested that the stale of HCV qua~species in NS2 region WSSlikelyto correlatewith the progression ofliver diseas

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    Preliminary Study of Hepatitis C Viral Quasispecies in NS2 Region in Patients wiUl Hepatitis C Virus Infection

    • 1. 1.华中科技大学同济医学院附属同济医院分子医学中心.湖北武汉43/}030
    • 2. 华中科技大学同济医学院基础医学研究所
    • 3. 军事医学科学院微生物流行病研究所,北京100071

    Abstract: To explore the genetic variation of nonstmetural(NS)2 region and its gene structural characteristics in hepatitis C vims quasispecies,NS2 genes ofHCV from a HCV as)~nptomatie~[Tier and apatien twith ab. nomud ALT level tteile amphfied by RT-nested-PCR respectively and cloned into T Yector.5 positive clones form each of them were selected~ domly for sequencing.Eve~-clone WaS unique in nuclectide sequence and amino acid sequence.A 0Bt all the NS2 gene clones from the asymptomatic carrier had complete ORF ex。ept OIle done had a stop codon at 835aain HCV polypmtein.Butwhenit ean3~tothepatientwith abnorm al ALT level,3 clones of them had stop cadom.at 835aa and 1 clone had a stop ced on at 887aa in polypretein. 0nly 1 clone had complete ORF.Comparing this ORF with the s~ ence of the as)~lptomatic carrier,most of the~aino acid changes were confined to the N-termlnai.Simulating the so。ondarv strucRu~s of Ns2 proteins with intact openreadingframe demomUated thatthe structure ofthe HCV patientwith abnormalA levelwas similart0 the dominant structure ofthe asymptomatic carrier.It could beseenfrom thetwopatientswith HCV infection thatHepatitis C viral quasispeciesin NS2 r 0nhadan obvious diversity between HCv as)~lptoraatic p~riier and HCV patient with abnomaal A leve1.The diversity suggested that the stale of HCV qua~species in NS2 region WSSlikelyto correlatewith the progression ofliver diseas

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