Citation: LIU Jian—sheng, LIU-Xin, XU Dong—lei, SHAO Cong—wen, HOU Zong—li. Genetic Analysis of Simian virus 40 from Peripheral Blood and Kidney of M acaque M onkeys .VIROLOGICA SINICA, 2003, 18(1) : 79-81.

Genetic Analysis of Simian virus 40 from Peripheral Blood and Kidney of M acaque M onkeys

  • Available online: 02 February 2003
  • In order to investigate the detection methods and gene structure of SV40 ST-ag and LT—ag— C in peripheral blood mononuclear cells (PBMC) and kidney cells from different Macaque monkeys, using PCR, 4 positive templates were obtained firstly, then 4 ST—ag gene fragments and 4 LT—ag—C gene fragments were achieved and sequenced. These antigens coding region of each isolate contain a number of nucleotide differences when compared to the reference strain SV40——776 and the laboratory strain SV40一B.These resuhs indicated that:1)SV40 DNA isolated from the PBMC and kidney cells showed essentially identical sequences for ST—ag gene and LT—ag—C gene. 21 the presence of viral DNA in P—BMC suggests that virus may spread with in the host by hematogenous routes. 3) a new SV40 strain predominates in our monkey population, it contains a specific nucleotide and amino acid changes in LT-ag—C termini, TCCTCACAG polynucleotide is inserted among nt2784/2783, SO SerSerGIn iS inserted after amino acid 678 residue.

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    Genetic Analysis of Simian virus 40 from Peripheral Blood and Kidney of M acaque M onkeys

    • 1. Chinese Academy ofMed&d Science,Peking Union Med&d University,Institute ofMed&d Biology,Kunming 650118, China

    Abstract: In order to investigate the detection methods and gene structure of SV40 ST-ag and LT—ag— C in peripheral blood mononuclear cells (PBMC) and kidney cells from different Macaque monkeys, using PCR, 4 positive templates were obtained firstly, then 4 ST—ag gene fragments and 4 LT—ag—C gene fragments were achieved and sequenced. These antigens coding region of each isolate contain a number of nucleotide differences when compared to the reference strain SV40——776 and the laboratory strain SV40一B.These resuhs indicated that:1)SV40 DNA isolated from the PBMC and kidney cells showed essentially identical sequences for ST—ag gene and LT—ag—C gene. 21 the presence of viral DNA in P—BMC suggests that virus may spread with in the host by hematogenous routes. 3) a new SV40 strain predominates in our monkey population, it contains a specific nucleotide and amino acid changes in LT-ag—C termini, TCCTCACAG polynucleotide is inserted among nt2784/2783, SO SerSerGIn iS inserted after amino acid 678 residue.

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