Citation: WANG Bin, LI Xia, YANG Xi-dong. Examination ofSpherical Viruses from Mytilus edulis .VIROLOGICA SINICA, 2003, 18(3) : 288-291.

Examination ofSpherical Viruses from Mytilus edulis

  • Available online: 18 June 2003
  • Abstract:A lot of spherical virions were observed in internal organiT.ation such as gill and mantellum of the diseased Mytilus edulis with negative staining method.The diameter of V on is around 8()_一 200nm with dual unit membran e.Sam e virions were observed in connective tissuese of infected organ s with ultrathin section electronmicroscopic observ ation.Th e“occlusion body’’were observed in cell matrix an d no inclusion bod y was present.A few same virions were observed in internal organization of normal Ruditapes variega~,Neptunea cumingi an d Dstrea lalienwhanensis with negative staining method.,nle normal Chlorostoma rusticum Can be infected by organizational liquid with the viruses from diseased Mytilus edulis through mouth and injured section on skin.Th e virus was purified from the diseased tissue by sucrose gradient centrifugation with 25 0oo r/min.3h.Th e virus sediment lies between 2O% ~ 3O% of sucrose. Key word:Mytilus edulis;SphericalAbstract:A lot of spherical virions were observed in internal organiT.ation such as gill and mantellum of the diseased Mytilus edulis with negative staining method.The diameter of V on is around 8()_一 200nm with dual unit membran e.Sam e virions were observed in connective tissuese of infected organ s with ultrathin section electronmicroscopic observ ation.Th e“occlusion body’’were observed in cell matrix an d no inclusion bod y was present.A few same virions were observed in internal organization of normal Ruditapes variega~,Neptunea cumingi an d Dstrea lalienwhanensis with negative staining method.,nle normal Chlorostoma rusticum Can be infected by organizational liquid with the viruses from diseased Mytilus edulis through mouth and injured section on skin.Th e virus was purified from the diseased tissue by sucrose gradient centrifugation with 25 0oo r/min.3h.Th e virus sediment lies between 2O% ~ 3O% of sucrose.

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    Examination ofSpherical Viruses from Mytilus edulis

    • 1. Key Laboratory of Mariculture Ecology,Dalian Fisheries College,Dalian 116024,China

    Abstract: Abstract:A lot of spherical virions were observed in internal organiT.ation such as gill and mantellum of the diseased Mytilus edulis with negative staining method.The diameter of V on is around 8()_一 200nm with dual unit membran e.Sam e virions were observed in connective tissuese of infected organ s with ultrathin section electronmicroscopic observ ation.Th e“occlusion body’’were observed in cell matrix an d no inclusion bod y was present.A few same virions were observed in internal organization of normal Ruditapes variega~,Neptunea cumingi an d Dstrea lalienwhanensis with negative staining method.,nle normal Chlorostoma rusticum Can be infected by organizational liquid with the viruses from diseased Mytilus edulis through mouth and injured section on skin.Th e virus was purified from the diseased tissue by sucrose gradient centrifugation with 25 0oo r/min.3h.Th e virus sediment lies between 2O% ~ 3O% of sucrose. Key word:Mytilus edulis;SphericalAbstract:A lot of spherical virions were observed in internal organiT.ation such as gill and mantellum of the diseased Mytilus edulis with negative staining method.The diameter of V on is around 8()_一 200nm with dual unit membran e.Sam e virions were observed in connective tissuese of infected organ s with ultrathin section electronmicroscopic observ ation.Th e“occlusion body’’were observed in cell matrix an d no inclusion bod y was present.A few same virions were observed in internal organization of normal Ruditapes variega~,Neptunea cumingi an d Dstrea lalienwhanensis with negative staining method.,nle normal Chlorostoma rusticum Can be infected by organizational liquid with the viruses from diseased Mytilus edulis through mouth and injured section on skin.Th e virus was purified from the diseased tissue by sucrose gradient centrifugation with 25 0oo r/min.3h.Th e virus sediment lies between 2O% ~ 3O% of sucrose.

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