Citation: ZHAO W en—hua, SONG Jian—ling, ZHU Jian—bo, XIN Ai—guo, WANG Jin—ping, LI Zhi—hua, ZHANG Nian—ZU. Sequencing and Analysis on the F and HN Genes of Avian paramyxovirus Type I Isolated from Parrot .VIROLOGICA SINICA, 2003, 18(4) : 394-396.

Sequencing and Analysis on the F and HN Genes of Avian paramyxovirus Type I Isolated from Parrot

  • Available online: 05 August 2003
  • Virion RNA was abstracted from purified type I Avian paramyxovirus strain YN—PA0 1 (isolated from parrot)and used as a template.The fragment containing the fusion gene(F)and hemagglutinin-·neuraminidase gene(HN)of the isolate was amplified by RT-·PCR and cloned to the pMD 1 8一T Vector.Using primer walking method the complete sequence of F-HN genes was obtained finally. And the respective amino acid sequence was deduced.Through relative software the phylogenetic trees on F gene an d HN gene were constructed between strain YN—PA0 1 an d reference strains.The results showed that strain YN—PA01 comparing with reference strains displays 98.7% -83.2% nucleotide homology an d 98.1%-86.2% amino acid homology on F gene;97.4%-79.1% nucleotide homology an d 97.2% -83.2% amino acid homology on HN gene.Additional 6 amino acids are encoded by the HN gene ORF of strain YN—PA01 comparing with national reference strains.Th e studied strain YN—PA01 exhibits highest identities with strain JS/5/0 1/Go either an alyzed on F gene or HN gene.

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    Sequencing and Analysis on the F and HN Genes of Avian paramyxovirus Type I Isolated from Parrot

    • 1. Yunnan Province Tropical and Subtropical Animal Virus Disease Laboratory,Kunming 650224,China

    Abstract: Virion RNA was abstracted from purified type I Avian paramyxovirus strain YN—PA0 1 (isolated from parrot)and used as a template.The fragment containing the fusion gene(F)and hemagglutinin-·neuraminidase gene(HN)of the isolate was amplified by RT-·PCR and cloned to the pMD 1 8一T Vector.Using primer walking method the complete sequence of F-HN genes was obtained finally. And the respective amino acid sequence was deduced.Through relative software the phylogenetic trees on F gene an d HN gene were constructed between strain YN—PA0 1 an d reference strains.The results showed that strain YN—PA01 comparing with reference strains displays 98.7% -83.2% nucleotide homology an d 98.1%-86.2% amino acid homology on F gene;97.4%-79.1% nucleotide homology an d 97.2% -83.2% amino acid homology on HN gene.Additional 6 amino acids are encoded by the HN gene ORF of strain YN—PA01 comparing with national reference strains.Th e studied strain YN—PA01 exhibits highest identities with strain JS/5/0 1/Go either an alyzed on F gene or HN gene.

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