Citation: XU Li, WANG Ling, SU Xin, WAN G, W U, CHEN Pu. Rapid M olecular Diagnosis of Concurrent Infection of Porcine Reproductive and Respiratory Syndrome an d Pseudorabies .VIROLOGICA SINICA, 2003, 18(6) : 614-615.

Rapid M olecular Diagnosis of Concurrent Infection of Porcine Reproductive and Respiratory Syndrome an d Pseudorabies

  • Available online: 25 December 2003
  • Abstract:According to the nucleotide sequences of Porcine Reproductive and Respiratory Synd rome V/ms VR2332 strain and Pseudorabies virus shan ghai strain provided in GenBank, two pairs of primersweredesignedto amplifytheN geneofPRRSV byRT—PCR an dTkgeneofPRV byPCR. As a result, a rapid molecular diagn osis with high specificity an d accuracy was set up.The result indicated that PRRSV Was detected from 11 out of 33 samples, PRV was detected from 9 out of 33 samples, and CO—infec tion by PRRSV an d PRV was confirmed in 8 samples, the rate of co-infection Was at24.2% .

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    Rapid M olecular Diagnosis of Concurrent Infection of Porcine Reproductive and Respiratory Syndrome an d Pseudorabies

    • 1. 1.KeyLaboratoryofAnimalDiseaseDiagnosisandImmunologyNanjingAgricultural University,Nanjing 210095. China
    • 2. Department ofAnimal Science.Agricultural College ofNingxia University.Yinchuan 750105.China 3 Animal Quarantine Institute of Agriculture Ministry,Qingdao 266032,China

    Abstract: Abstract:According to the nucleotide sequences of Porcine Reproductive and Respiratory Synd rome V/ms VR2332 strain and Pseudorabies virus shan ghai strain provided in GenBank, two pairs of primersweredesignedto amplifytheN geneofPRRSV byRT—PCR an dTkgeneofPRV byPCR. As a result, a rapid molecular diagn osis with high specificity an d accuracy was set up.The result indicated that PRRSV Was detected from 11 out of 33 samples, PRV was detected from 9 out of 33 samples, and CO—infec tion by PRRSV an d PRV was confirmed in 8 samples, the rate of co-infection Was at24.2% .

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