Citation: JIN Ye, LU Cheng-ping, LI Xian. Property of Viral Nucleic Acid from the Virus Causing the Trembling disease in Eriocheir sinensis .VIROLOGICA SINICA, 2004, 19(1) : 36-38.

Property of Viral Nucleic Acid from the Virus Causing the Trembling disease in Eriocheir sinensis

  • Corresponding author: LU Cheng-ping, 
  • Available online: 20 February 2004
  • In this paper we reported the electron microscope(EM) examination of “Trembling disease” virus in Eriocheir sinensis. The virions were globular, with envelope and spikes, and about 150nm of diameter. The purified nucleic acid was treated with 8mol/L urea and the viral nucleic acid was transferred to the EM grids by aqueous technique. The viral nucleic acid was single stranded under EM observation. The molecular weight of the viral nucleic was calculated to be about 5.05×106. The agrosegel electrophoresis of the purified viral nucleic acid showed only one band, with the size of about 15~16kb. The nucleic acid was sensitive to RNase and Mung Bean Nuclease treatment, but not sensitive to Dnase I. The above data demonstrated that the nucleic acid should be a ssRNA.

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    Property of Viral Nucleic Acid from the Virus Causing the Trembling disease in Eriocheir sinensis

      Corresponding author: LU Cheng-ping,
    • 1. 1. Key Lab of Animal Disease Diagnostic and Immunology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China
    • 2. Jiangsu Province Center for Disease Prevention and Control, Nanjing 210009, China

    Abstract: In this paper we reported the electron microscope(EM) examination of “Trembling disease” virus in Eriocheir sinensis. The virions were globular, with envelope and spikes, and about 150nm of diameter. The purified nucleic acid was treated with 8mol/L urea and the viral nucleic acid was transferred to the EM grids by aqueous technique. The viral nucleic acid was single stranded under EM observation. The molecular weight of the viral nucleic was calculated to be about 5.05×106. The agrosegel electrophoresis of the purified viral nucleic acid showed only one band, with the size of about 15~16kb. The nucleic acid was sensitive to RNase and Mung Bean Nuclease treatment, but not sensitive to Dnase I. The above data demonstrated that the nucleic acid should be a ssRNA.

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