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Volume 19 Issue 2
April 2004
    Citation: WU Ying-yun, HONG Kun-xue, CHEN Pei, CHEN Qi-ming, GENG Yun-qi, SHAO Yi-ming. Generation of Full-genome SHIV-KB9 Clone and Its Replication in Human and Monkey Peripheral Blood Mononuclear Cells* .VIROLOGICA SINICA, 2004, 19(2) : 114-119.
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    Generation of Full-genome SHIV-KB9 Clone and Its Replication in Human and Monkey Peripheral Blood Mononuclear Cells*

    • 1.

      1. College of Life Sciences, Nankai University, Tianjin 300071, China

    • 2.

      National Center for AIDS/STD Control and Prevention, Beijing 100050, China

    • Corresponding author: SHAO Yi-ming, 
    • Available online: 20 April 2004
    • SHIV-KB9 chimeric virus was constructed by two constructs representing the 3’ and 5’ fragments of the viral genome. This full-genome SHIV-KB9, with large size and long terminal repeat sequence (LTR), could be maintained stable in E.coli JM109 strain in the cultivation condition of 30℃ and 180r/min. The infectivity of the full-length SHIV-KB9 in human peripheral blood mononuclear cells (PBMC), cynomolgus and rhesus monkey PBMC was characterized by polymerase chain reaction (PCR), reverse transcription PCR (RT-PCR) and Simian immunodeficiency virus (SIV) p27 gag antigen titration assay.
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      Generation of Full-genome SHIV-KB9 Clone and Its Replication in Human and Monkey Peripheral Blood Mononuclear Cells*

        Corresponding author: SHAO Yi-ming,
      • 1. 1. College of Life Sciences, Nankai University, Tianjin 300071, China
      • 2. National Center for AIDS/STD Control and Prevention, Beijing 100050, China

      Abstract: SHIV-KB9 chimeric virus was constructed by two constructs representing the 3’ and 5’ fragments of the viral genome. This full-genome SHIV-KB9, with large size and long terminal repeat sequence (LTR), could be maintained stable in E.coli JM109 strain in the cultivation condition of 30℃ and 180r/min. The infectivity of the full-length SHIV-KB9 in human peripheral blood mononuclear cells (PBMC), cynomolgus and rhesus monkey PBMC was characterized by polymerase chain reaction (PCR), reverse transcription PCR (RT-PCR) and Simian immunodeficiency virus (SIV) p27 gag antigen titration assay.

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