Expression and Immunologenic Study of the Hantaan Virus Glycoprotein G1 and G2 by Using the Adenoviral Vector
Abstract: In order to study the immune responses of Hantaan virus envelope glycoproteins G1 and G2 in adenovirus expression system, G1 and G2 gene were amplified from Hantaan virus 76-118 and cloned to the plasmid pAdTrackCMV. Positive clones pAdTrackCMV-G1 and G2 were determined with restriction enzyme digestion. The recombinant adenovirus rAdeasy-G1 and rAdeasy-G2 were generated by bacterial homologous recombination with adenoviral plasmid pAdeasy-1 in E.coli BJ5183. After amplification in the 293 cell, the recombinant viruses were immuned to the Balb/c mice. The antibody reaction were detected by ELISA and Immunofluorescence Assay. Six rAdeasy-G1 immuned mice and four rAdeasy-G2 immuned mice produce antibody which can specifically react with the Hantaan virus. These results suggested a possible application of adenovir- us expression system.