Citation: LI Xiao-qing, ZHOU Rui, JIA Yun-li, JIA Fang, YU Ze-hua, CHEN Xin-wen. Function of Actin in Transportation of the AcMNPV from Nuclear to out side of Cell .VIROLOGICA SINICA, 2004, 19(6) : 630-635.

Function of Actin in Transportation of the AcMNPV from Nuclear to out side of Cell

  • Corresponding author: YU Ze-hua, 
  • Available online: 20 December 2004
  • To investigate the role of actin in AcMNPV BV transportation from nuclear to cytoplasm, we constructed two recombinant viruses AcMNPV-GFP and AcMNPV-GFP-actin, in which the expression of GFP gene and GFP-actin fusion gene was driven by polyhedrin promoter. Here, we report the expression pattern of GFP in Sf9 cells infected with AcMNPV-GFP and AcMNPV-GFP-actin. Within 24-72 hr after infection, we observed that GFP signal in Sf9 cells infected with AcMNPV-GFP-actin was first concentrated in nuclear and then gradually located to cytoplasm and finally accumulated exclusively at cytoplasmic membrane. In the Sf9 cells infected with AcMNPV-GFP, however, the GFP signal was diffused throughout cell and this expression pattern did not change during the same period of observation. Thus, our results indicated that actin might participate in the transportation of the AcMNPV BV from nuclear to cytoplasm and its exocytosis from the cell.

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    Function of Actin in Transportation of the AcMNPV from Nuclear to out side of Cell

      Corresponding author: YU Ze-hua,
    • 1. 1. lnstitute of Entomology, Central China Normal University, Wuhan 430079, China
    • 2. Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China

    Abstract: To investigate the role of actin in AcMNPV BV transportation from nuclear to cytoplasm, we constructed two recombinant viruses AcMNPV-GFP and AcMNPV-GFP-actin, in which the expression of GFP gene and GFP-actin fusion gene was driven by polyhedrin promoter. Here, we report the expression pattern of GFP in Sf9 cells infected with AcMNPV-GFP and AcMNPV-GFP-actin. Within 24-72 hr after infection, we observed that GFP signal in Sf9 cells infected with AcMNPV-GFP-actin was first concentrated in nuclear and then gradually located to cytoplasm and finally accumulated exclusively at cytoplasmic membrane. In the Sf9 cells infected with AcMNPV-GFP, however, the GFP signal was diffused throughout cell and this expression pattern did not change during the same period of observation. Thus, our results indicated that actin might participate in the transportation of the AcMNPV BV from nuclear to cytoplasm and its exocytosis from the cell.

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