The Characteristics of Pseudorabies Virus Gene-deleted Vaccine Strain

In order to determine the effect of deletion of gE, gI and TK on the characteristics of virion, we studied the property in cell culture, physicochemical characters and the morphogenesis in vero cells of Pseudorabies virus(PRV) gene-deleted vaccine strain SA215. The results indicated that PRV SA215 had the similar process of adsorption and penetration kinetics as its parental strain Fa; however, the course of replication was prolonged. Furthermore, the deletion of gE, gI and TK only has slight effect on physicochemical characters. The comparative analysis of SA215 and Fa on the morphogenesis in cultured cells suggested that the process of budding from nucleus membrane and forming of secondary envelope were delayed or arrested. The results provide guidance for manufacture of PRV gene-deleted vaccine SA215.