Prophylaxtic Efficacy of Recombinant Fowl-pox Virus and DNA Vaccine Against Foot-and-Mouth Disease Virus in Guinea Pigs

A recombinant Fowlpox virus (vUTAL3CP1) containing FMDV capsid, P1-2A, and viral 3C protease coding regions,and another plasmid DNA (pVIRIL18P1) encoding P1-2A gene and swine interleukin-18 (IL-18) cDNA,were constructed. guinea pigs were inoculated intramuscularly twice at a 28-day interval with vUTAL3CP1 and pVIRIL18P1 alone, respectively, or vUTAL3CP1 priming and pVIRIL18P1 boosting. To evaluate the prophylaxtic efficacy of these vaccine candidates, anti-FMDV antibodies, neutralizing antibodies and T cell proliferation were detected. All of Guinea pigs were challenged with 250ID_{50} FMDV. The results indicated that both vaccines could elicit specific humoral immune and cellular immune responses. Compared with conventional inactivated vaccines, vUTAL3CP1 elicited not only similar FMDV antibody level but also stronger T cell proliferation. Three in four guinea pigs immunized by vUTAL3CP1 were protected completely from the FMDV infection, while other recombinant vaccines inoculated groups had much lower prote