Sequence Analysis of RpL 13 Gene from HzAm1 Cells and Effect of HaSNPV Infection on the Transcription of RpL13
Abstract: The cDNA and genomic DNA of ribosomal protein L13 (RpL13) gene were cloned from Helicoverpa zea cells(HzAM1). Sequence analysis indicated that the open reading frame of rpL13 was composed of 666bp without intron,encoding a protein of 25 kDa. The sequence was used to construct phylogentic trees with other known sequences of 15 animal rpL13s. The result suggested it has similar topology with the classical taxonomy. Quantitative RT-PCR assay revealed that the transcription level of rpL13 declined in the HzAm1 cells infected by Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus (HaSNPV). At 96 hours post infection, the mRNA copies of rpL13 were about 8% to that of healthy cells.