Citation: TANG Jing-yuan, JIANG Ping*, JIANG Wen-ming, MA Su, LI Yu-feng. Construction and Immunogenicity of Recombinant Adenovirus Expressing the M protein of Porcine Re- productive and Respiratory Syndrome Virus in Mice .VIROLOGICA SINICA, 2005, 20(6) : 618-622.

Construction and Immunogenicity of Recombinant Adenovirus Expressing the M protein of Porcine Re- productive and Respiratory Syndrome Virus in Mice

  • Available online: 20 December 2005
  • To screen out vaccine candidate against PRRSV, adenovirus vector was used to express the M protein of Porcine reproductive and respiratory syndrome virus (PRRSV). A recombinant adenovirus was constructed and the expression of the M protein was identified by RT-PCR and indirect immunofluorescence assay (IFA). The purified recombinant adenovirus M (rAd-M) was passaged 25 times in 293A cells. The titer of stocks of rAd-M was stably 107.8\CID50/mL. Furthermore, the recombinant M protein adenovirus could induce PRRSV specific humoral immunity and cell mediated immunity in mice. It indicated that the recombinant adenovirus expressing the main structural proteins of PRRSV is a potentially viable candidate vaccine against PRRSV.

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    Construction and Immunogenicity of Recombinant Adenovirus Expressing the M protein of Porcine Re- productive and Respiratory Syndrome Virus in Mice

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    Abstract: To screen out vaccine candidate against PRRSV, adenovirus vector was used to express the M protein of Porcine reproductive and respiratory syndrome virus (PRRSV). A recombinant adenovirus was constructed and the expression of the M protein was identified by RT-PCR and indirect immunofluorescence assay (IFA). The purified recombinant adenovirus M (rAd-M) was passaged 25 times in 293A cells. The titer of stocks of rAd-M was stably 107.8\CID50/mL. Furthermore, the recombinant M protein adenovirus could induce PRRSV specific humoral immunity and cell mediated immunity in mice. It indicated that the recombinant adenovirus expressing the main structural proteins of PRRSV is a potentially viable candidate vaccine against PRRSV.

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