For best viewing of the website please use Mozilla Firefox or Google Chrome.
Citation: CUN Wei, MA Shao-hui, ZHAO Hong-ling, LIU Long-ding, WANG Li-chun, DONG Cheng-Hong, ZHANG Ying, WANG Xiang, LI Qi-han*, . Localization of HSV-I ICP22 Protein in Cells by Antibody Against a predicted Antigenic Peptide [J].VIROLOGICA SINICA, 2006, 21(3) : 226-230.

Localization of HSV-I ICP22 Protein in Cells by Antibody Against a predicted Antigenic Peptide

  • As one of the viral proteins expressed in the immediate-early phase of HSVI infection, ICP22 functions in the regulation of viral replication. However, it is difficult to produce specific antibody against ICP22 protein with homology of antigenicity in this protein. Based upon prediction of antigenicity of ICP22, a peptide of 1-36 amino acids was thought to possess a specific antigenic epitope, which was expressed as a fusion protein with GST. The antibody, raised in mice immunized with a purified fusion protein, was able to recognize ICP22 expressed in eukaryotic cells. Analysis with this antibody suggested a nuclear locatization of ICP22 and specific punctuative structure in cells.

  • 加载中
  • 加载中

Article Metrics

Article views(2677) PDF downloads(1308) Cited by()

Related
Proportional views
    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Localization of HSV-I ICP22 Protein in Cells by Antibody Against a predicted Antigenic Peptide

    • 1. Institute of Medical Biology,Chinese Academemy of Medical Sciences and PekingUnion Medical college, Kunming 650118,China

    Abstract: As one of the viral proteins expressed in the immediate-early phase of HSVI infection, ICP22 functions in the regulation of viral replication. However, it is difficult to produce specific antibody against ICP22 protein with homology of antigenicity in this protein. Based upon prediction of antigenicity of ICP22, a peptide of 1-36 amino acids was thought to possess a specific antigenic epitope, which was expressed as a fusion protein with GST. The antibody, raised in mice immunized with a purified fusion protein, was able to recognize ICP22 expressed in eukaryotic cells. Analysis with this antibody suggested a nuclear locatization of ICP22 and specific punctuative structure in cells.

    Relative (20)

    目录

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return