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Volume 21 Issue 5
October 2006
    Citation: JI Xiang-bo, LIU Wen-bo, WEI Jian-chao, NIU Ming-fu, ZHOU-bin, CAO Rui-bing, HEN Pu-yan*. Construction and Immune Effect of the Recombinant DNA Vaccine Expressing ILTV Major Antigen Epitope gene .VIROLOGICA SINICA, 2006, 21(5) : 481-484.
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    Construction and Immune Effect of the Recombinant DNA Vaccine Expressing ILTV Major Antigen Epitope gene

    • 1.

      Key laboratory of animal disease diagnostic and immunology, Ministry of agricultural science, Nanjing Agricultural University, Nanjing 210095

    • Available online: 20 September 2006
    • A Plasmid DNA vaccine pcDNA-gB expressing gB protein of infectious laryngotracheitis virus(ILTV) Yantai strain was constructed. The expression of viral protein from constructed DNA vaccine was confirmed by an in vitro transcription /translation system and transtection in 293-T cells. To evaluate the prophylactic efficacy of the DNA vaccine, 4-week-old chickens were inoculated with pcDNA-gB plasmid and rFPV- gB-gD-IgY, respectively followed by a booster of rFPV-gB-gD-IgY and pcDNA-gB. Anti-ILTV antibodies and T cell proliferation were detected. The results indicated that both vaccines could elicit specific humeral immune and cellular immune responses. Compared with attenuated vaccines, rFPV-gB-gD-IgY/pcDNA-gB elicited not only similar antibody level of ILTV but stronger T cell proliferation．The study lays foundation on further investigation for the new type of vaccines against ILTV.
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      Construction and Immune Effect of the Recombinant DNA Vaccine Expressing ILTV Major Antigen Epitope gene

      • 1. Key laboratory of animal disease diagnostic and immunology, Ministry of agricultural science, Nanjing Agricultural University, Nanjing 210095

      Abstract: A Plasmid DNA vaccine pcDNA-gB expressing gB protein of infectious laryngotracheitis virus(ILTV) Yantai strain was constructed. The expression of viral protein from constructed DNA vaccine was confirmed by an in vitro transcription /translation system and transtection in 293-T cells. To evaluate the prophylactic efficacy of the DNA vaccine, 4-week-old chickens were inoculated with pcDNA-gB plasmid and rFPV- gB-gD-IgY, respectively followed by a booster of rFPV-gB-gD-IgY and pcDNA-gB. Anti-ILTV antibodies and T cell proliferation were detected. The results indicated that both vaccines could elicit specific humeral immune and cellular immune responses. Compared with attenuated vaccines, rFPV-gB-gD-IgY/pcDNA-gB elicited not only similar antibody level of ILTV but stronger T cell proliferation．The study lays foundation on further investigation for the new type of vaccines against ILTV.

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