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Volume 25 Issue 2
April 2010
    Citation: Ruo-feng SHANG, Jian-ping LIANG, Zhong-yuan NA, Hong-jun YANG, Yu LU, Lan-ying HUA, Wen-zhu GUO, Ying CUI, Ling WANG. In vivo Inhibition of NAS Preparation on H9N2 Subtype AIV* .VIROLOGICA SINICA, 2010, 25(2) : 145-150.  http://dx.doi.org/10.1007/s12250-010-3082-8
    shu

    In vivo Inhibition of NAS Preparation on H9N2 Subtype AIV*

    cstr: 32224.14.s12250-010-3082-8
    • 1.

      Key Laboratory of New Animal Medicine Project, Chinese Academy of Agricultural Sciences, Lanzhou Insititute of Animal Husbandry & Veterinary Pharamaceutics Science, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China

    • 2.

      Yunnan Eco-agricultural Research Institute, Kunming 650106, China

    • Corresponding author: Jian-ping LIANG, Liangjp100@sina.com
    • Received Date: 10 June 2009
      Accepted Date: 12 January 2010
      Available online: 01 April 2010

      Fund Project: Key Technologies Research and Development Program 2004BA519A26

    • NAS preparation, a kind of Chinese herbal medicine found by the Yunnan Eco-agricultural Research Institute, has potential antiviral activity. In this paper, the inhibiting effect of NAS preparation on H9N2 subtype Avian influenza virus (AIV) was investigated in vivo. Chickens infected with H9N2 virus were treated with NAS preparation for 4 days. The virus was then detected by hemoagglutination (HA) test and reverse transcription polymerase chain reaction (RT-PCR). The results showed that no H9N2 virus could be detected at the 7th day when the chickens were treated with 0.2g/kg/d or 0.1g/kg/d of NAS preparation. However the virus could be detected in other chickens without NAS preparation treatment. This result suggested that NAS preparation may be a potential drug candidate to control infection of H9N2 subtype AIV in chickens.
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      8. Kadir T, Kyosuke N, Avin K, et al. 1996. Antiviral effect of Sanicula europaea L. leaves extract on influenza virus-infected cells. Biochem Biophys Res Commun, 225:22-26.
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      9. Katz J M, Lu X H, Tumpey T M, et al. 2000. Molecular correlates of influenza A H5N1 virus pathogenesis in mice. J Virol, 74(22): 10807-10810.
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      In vivo Inhibition of NAS Preparation on H9N2 Subtype AIV*

        Corresponding author: Jian-ping LIANG, Liangjp100@sina.com
      • 1. Key Laboratory of New Animal Medicine Project, Chinese Academy of Agricultural Sciences, Lanzhou Insititute of Animal Husbandry & Veterinary Pharamaceutics Science, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China
      • 2. Yunnan Eco-agricultural Research Institute, Kunming 650106, China
      • Received Date: 10 June 2009
      • Accepted Date: 12 January 2010
      Fund Project:  Key Technologies Research and Development Program 2004BA519A26

      Abstract: NAS preparation, a kind of Chinese herbal medicine found by the Yunnan Eco-agricultural Research Institute, has potential antiviral activity. In this paper, the inhibiting effect of NAS preparation on H9N2 subtype Avian influenza virus (AIV) was investigated in vivo. Chickens infected with H9N2 virus were treated with NAS preparation for 4 days. The virus was then detected by hemoagglutination (HA) test and reverse transcription polymerase chain reaction (RT-PCR). The results showed that no H9N2 virus could be detected at the 7th day when the chickens were treated with 0.2g/kg/d or 0.1g/kg/d of NAS preparation. However the virus could be detected in other chickens without NAS preparation treatment. This result suggested that NAS preparation may be a potential drug candidate to control infection of H9N2 subtype AIV in chickens.

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