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Volume 25 Issue 3
June 2010
    Citation: LI Jian-qiang, CHENG Jie, LAN Xi, LI Xue-rui, LI Wei, YIN Xiang-ping, LI Bao-yu, YANG Bin, LI Zhi-yong, ZHANG Yun, LIU Ji-xing. Complete Genomic Sequence of Transmissible Gastroenteritis Virus TS and 3o End Sequence Characterization Following Cell Culture .VIROLOGICA SINICA, 2010, 25(3) : 213-224.  http://dx.doi.org/10.1007/s12250-010-3108-2
    shu

    Complete Genomic Sequence of Transmissible Gastroenteritis Virus TS and 3o End Sequence Characterization Following Cell Culture

    • 1.

      Key laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China

    • 2.

      Lanzou Institute of Biological Products, Lanzhou 730070, China

    • Corresponding author: LIU Ji-xing, liujixing@hotmail.com
    • Received Date: 29 October 2009
      Accepted Date: 14 April 2010
      Available online: 01 June 2010

      Fund Project: National Basic Research Program (2004CCA00500) 2004CCA00500National High-tech Development Research Program of China 2006AA02Z440

    • The complete genome sequence of transmissible Gastroenteritis virus (TGEV) strain TS, previously isolated from Gansu province, was cloned and compared with published sequence data from other TGEV strains. Phylogenetic tree analysis based on the amino acid and nucleotide sequences of the S gene showed that the TGEV strains were divided into 3 clusters. TGEV TS showed a close evolutionary relationship to the American Miller cluster but had a 5’ non-translated region (NTR) sequence closely related to the American Purdue cluster. Continued culture in different cell types indicated that TGEV TS virulence could be attenuated after fifty passages in Porcine kidney (PK-15) cells, and that the Porcine kidney cell line IB-RS-2 (IBRS) was not suitable for culture of the TGEV strain TS.
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      Complete Genomic Sequence of Transmissible Gastroenteritis Virus TS and 3o End Sequence Characterization Following Cell Culture

        Corresponding author: LIU Ji-xing, liujixing@hotmail.com
      • 1. Key laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
      • 2. Lanzou Institute of Biological Products, Lanzhou 730070, China
      • Received Date: 29 October 2009
      • Accepted Date: 14 April 2010
      Fund Project:  National Basic Research Program (2004CCA00500) 2004CCA00500National High-tech Development Research Program of China 2006AA02Z440

      Abstract: The complete genome sequence of transmissible Gastroenteritis virus (TGEV) strain TS, previously isolated from Gansu province, was cloned and compared with published sequence data from other TGEV strains. Phylogenetic tree analysis based on the amino acid and nucleotide sequences of the S gene showed that the TGEV strains were divided into 3 clusters. TGEV TS showed a close evolutionary relationship to the American Miller cluster but had a 5’ non-translated region (NTR) sequence closely related to the American Purdue cluster. Continued culture in different cell types indicated that TGEV TS virulence could be attenuated after fifty passages in Porcine kidney (PK-15) cells, and that the Porcine kidney cell line IB-RS-2 (IBRS) was not suitable for culture of the TGEV strain TS.

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