Citation: Jun Chen, Xiu Liang, Pei-fu Chen. Canine Distemper Virus Utilizes Different Receptors to Infect Chicken Embryo Fibroblasts and Vero cells* .VIROLOGICA SINICA, 2011, 26(2) : 139-145.  http://dx.doi.org/10.1007/s12250-011-3176-3

Canine Distemper Virus Utilizes Different Receptors to Infect Chicken Embryo Fibroblasts and Vero cells*

  • Corresponding author: Pei-fu Chen, cltwins2003@sina.com
  • Received Date: 05 December 2010
    Accepted Date: 15 February 2011
    Available online: 01 April 2011

    Fund Project: Yunnan Provincial Education Board 08C0070Yunnan Provincial Program for Introducing High-level Scientists 2009CI125

  • Inducing animal viruses to adapt to chicken embryos or chicken embryo fibroblasts (CEF) is a common method to develop attenuated live vaccines with full security. Canine distemper virus (CDV) also does this, but the mechanisms and particular receptors remain unclear. Virus overlay protein blot assays were carried out on CEF membrane proteins, which were extracted respectively with a Mem-PERTM kit, a radioimmunoprecipitation assay buffer or a modified co-immunoprecipitation method, and revealed a common 57 kDa positive band that differed from the 42-kDa positive band in Vero cells and also from those receptors reported in lymphocytes and 293 cells, indicating a receptor diversity of CDV and the possibility of the 57-kDa protein acting as a receptor that is involved in adaptive infection of CDV Kunming strain to CEF.

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    8. Singethan K, Topfstedt E, Schubert S, et al. 2006. CD9-dependent regulation of Canine distemper virus-induced cell-cell fusion segregates with the extracellular domain of the haemagglutinin. J Gen Virol, 87 (6): 1635-1642.
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    Canine Distemper Virus Utilizes Different Receptors to Infect Chicken Embryo Fibroblasts and Vero cells*

      Corresponding author: Pei-fu Chen, cltwins2003@sina.com
    • College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China
    Fund Project:  Yunnan Provincial Education Board 08C0070Yunnan Provincial Program for Introducing High-level Scientists 2009CI125

    Abstract: Inducing animal viruses to adapt to chicken embryos or chicken embryo fibroblasts (CEF) is a common method to develop attenuated live vaccines with full security. Canine distemper virus (CDV) also does this, but the mechanisms and particular receptors remain unclear. Virus overlay protein blot assays were carried out on CEF membrane proteins, which were extracted respectively with a Mem-PERTM kit, a radioimmunoprecipitation assay buffer or a modified co-immunoprecipitation method, and revealed a common 57 kDa positive band that differed from the 42-kDa positive band in Vero cells and also from those receptors reported in lymphocytes and 293 cells, indicating a receptor diversity of CDV and the possibility of the 57-kDa protein acting as a receptor that is involved in adaptive infection of CDV Kunming strain to CEF.