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Volume 26 Issue 2
April 2011
    Citation: Jian Qiu, Bo Qin, Simon Rayner, Chun-chen Wu, Rong-juan Pei, Song Xu, Yun Wang, Xin-wen Chen. Novel Evidence Suggests Hepatitis B Virus Surface Proteins Participate in Regulation of HBV Genome Replication* .VIROLOGICA SINICA, 2011, 26(2) : 131-138.  http://dx.doi.org/10.1007/s12250-011-3190-0
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    Novel Evidence Suggests Hepatitis B Virus Surface Proteins Participate in Regulation of HBV Genome Replication*

    cstr: 32224.14.s12250-011-3190-0
    • 1.

      State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China

    • 2.

      Graduate University of the Chinese Academy of Sciences, Beijing 100039, China

    • Corresponding author: Xin-wen Chen, wangyun@wh.iov.cn
    • These authors contributed equally to this work
    • Received Date: 14 February 2011
      Accepted Date: 02 March 2011
      Available online: 01 April 2011

      Fund Project: National Basic Research Program of China 2007CB512900

    • Naturally occurring mutations in surface proteins of Hepatitis B virus (HBV) usually result in altered hepatitis B surface antigen (HBsAg) secretion efficiency. In the present study, we reported two conserved residues, M75 and M103 with respect to HBsAg, mutations of which not only attenuated HBsAg secretion (M75 only), but also suppressed HBV genome replication without compromising the overlapping p-gene product. We also found M75 and M103 can initiate truncated surface protein (TSPs) synthesis upon over-expression of full-length surface proteins, which may possibly contribute to HBV genome replication. However, attempts to rescue replication- defective HBV mutant by co-expression of TSPs initiated from M75 or M103 were unsuccessful, which indicated surface proteins rather than the putative TSPs were involved in regulation of HBV genome replication.
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      8. Khan N, Guarnieri M, Ahn S H, et al. 2004. Modulation of hepatitis b virus secretion by naturally occurring mutations in the s gene. J Virol, 78 (7): 3262-3270.
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      11. Lin X, Yuan Z H, Wu L, et al. 2001. A single amino acid in the reverse transcriptase domain of hepatitis b virus affects virus replication efficiency. J Virol, 75 (23): 11827-11833.
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      12. Locarnini S. 2004. Molecular virology of hepatitis b virus. Semin Liver Dis, 24 Suppl 1: 3-10.

      13. Loffler-Mary H, Dumortier J, Klentsch-Zimmer C, et al. 2000. Hepatitis b virus assembly is sensitive to changes in the cytosolic s loop of the envelope proteins. Virology, 270 (2): 358-367.
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      14. Nishikawa M, Ryo K, Kimura T, et al. 1995. Mutant of the pre-s and s region of HBV. Nippon Rinsho, 53 Suppl (Pt 2): 47-51.

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      Novel Evidence Suggests Hepatitis B Virus Surface Proteins Participate in Regulation of HBV Genome Replication*

        Corresponding author: Xin-wen Chen, wangyun@wh.iov.cn
      • 1. State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
      • 2. Graduate University of the Chinese Academy of Sciences, Beijing 100039, China
      • Received Date: 14 February 2011
      • Accepted Date: 02 March 2011
      Fund Project:  National Basic Research Program of China 2007CB512900

      Abstract: Naturally occurring mutations in surface proteins of Hepatitis B virus (HBV) usually result in altered hepatitis B surface antigen (HBsAg) secretion efficiency. In the present study, we reported two conserved residues, M75 and M103 with respect to HBsAg, mutations of which not only attenuated HBsAg secretion (M75 only), but also suppressed HBV genome replication without compromising the overlapping p-gene product. We also found M75 and M103 can initiate truncated surface protein (TSPs) synthesis upon over-expression of full-length surface proteins, which may possibly contribute to HBV genome replication. However, attempts to rescue replication- defective HBV mutant by co-expression of TSPs initiated from M75 or M103 were unsuccessful, which indicated surface proteins rather than the putative TSPs were involved in regulation of HBV genome replication.

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