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Volume 29 Issue 4
August 2014
    Citation: Luisa Fernanda Mancipe Jiménez, Gloria Ramírez Nieto, Victor Vera Alfonso, Jairo Jaime Correa. Association of swine influenza H1N1 pandemic virus (SIVH1N1p) with porcine respiratory disease complex in sows from commercial pig farms in Colombia .VIROLOGICA SINICA, 2014, 29(4) : 242-249.  http://dx.doi.org/10.1007/s12250-014-3471-5
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    Association of swine influenza H1N1 pandemic virus (SIVH1N1p) with porcine respiratory disease complex in sows from commercial pig farms in Colombia

    cstr: 32224.14.s12250-014-3471-5

    • Laboratory of Animal Virology, Faculty of Veterinary Medicine and Animal Science, National University of Colombia, Bogotá D. C., Colombia

    • Corresponding author: Luisa Fernanda Mancipe Jiménez, lfmancipej@unal.edu.co
      Jairo Jaime Correa, jjaimec@unal.edu.co
    • Received Date: 13 May 2014
      Accepted Date: 01 August 2014
      Published Date: 08 August 2014
      Available online: 01 August 2014
    • Porcine respiratory disease complex (PRDC) is a serious health problem that mainly affects growing and finishing pigs. PRDC is caused by a combination of viral and bacterial agents, such as porcine reproductive and respiratory syndrome virus (PRRSV), swine influenza virus (SIV), Mycoplasma hyopneumoniae (Myh), Actinobacillus pleuropneumoniae (APP), Pasteurella multocida and Porcine circovirus 2 (PCV2). To characterize the specific role of swine influenza virus in PRDC presentation in Colombia, 11 farms from three major production regions in Colombia were examined in this study. Nasal swabs, bronchial lavage and lung tissue samples were obtained from animals displaying symptoms compatible with SIV. Isolation of SIV was performed in 9-day embryonated chicken eggs or Madin-Darby Canine Kidney (MDCK) cells. Positive isolates, identified via the hemagglutination inhibition test, were further analyzed using PCR. Overall, 7 of the 11 farms were positive for SIV. Notably, sequencing of the gene encoding the hemagglutinin (HA) protein led to grouping of strains into circulating viruses identified during the human outbreak of 2009, classified as pandemic H1N1-2009. Serum samples from 198 gilts and multiparous sows between 2008 and 2009 were obtained to determine antibody presence of APP, Myh, PCV2 and PRRSV in both SIV-H1N1p-negative and-positive farms, but higher levels were recorded for SIVH1N1p-positive farms. Odds ratio (OR) and P values revealed statistically significant differences (p<0.05) in PRDC presentation in gilts and multiparous sows of farms positive for SIV-H1N1p. Our findings indicate that positive farms have increased risk of PRDC presentation, in particular, PCV2, APP and Myh.
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      Association of swine influenza H1N1 pandemic virus (SIVH1N1p) with porcine respiratory disease complex in sows from commercial pig farms in Colombia

        Corresponding author: Luisa Fernanda Mancipe Jiménez, lfmancipej@unal.edu.co
        Corresponding author: Jairo Jaime Correa, jjaimec@unal.edu.co
      • Laboratory of Animal Virology, Faculty of Veterinary Medicine and Animal Science, National University of Colombia, Bogotá D. C., Colombia
      • Received Date: 13 May 2014
      • Accepted Date: 01 August 2014
      • Published Date: 08 August 2014

      Abstract: Porcine respiratory disease complex (PRDC) is a serious health problem that mainly affects growing and finishing pigs. PRDC is caused by a combination of viral and bacterial agents, such as porcine reproductive and respiratory syndrome virus (PRRSV), swine influenza virus (SIV), Mycoplasma hyopneumoniae (Myh), Actinobacillus pleuropneumoniae (APP), Pasteurella multocida and Porcine circovirus 2 (PCV2). To characterize the specific role of swine influenza virus in PRDC presentation in Colombia, 11 farms from three major production regions in Colombia were examined in this study. Nasal swabs, bronchial lavage and lung tissue samples were obtained from animals displaying symptoms compatible with SIV. Isolation of SIV was performed in 9-day embryonated chicken eggs or Madin-Darby Canine Kidney (MDCK) cells. Positive isolates, identified via the hemagglutination inhibition test, were further analyzed using PCR. Overall, 7 of the 11 farms were positive for SIV. Notably, sequencing of the gene encoding the hemagglutinin (HA) protein led to grouping of strains into circulating viruses identified during the human outbreak of 2009, classified as pandemic H1N1-2009. Serum samples from 198 gilts and multiparous sows between 2008 and 2009 were obtained to determine antibody presence of APP, Myh, PCV2 and PRRSV in both SIV-H1N1p-negative and-positive farms, but higher levels were recorded for SIVH1N1p-positive farms. Odds ratio (OR) and P values revealed statistically significant differences (p<0.05) in PRDC presentation in gilts and multiparous sows of farms positive for SIV-H1N1p. Our findings indicate that positive farms have increased risk of PRDC presentation, in particular, PCV2, APP and Myh.

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