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Volume 31 Issue 5
October 2016
    Citation: Hailiang Sun, Jian-Li Xue, Elizabeth Bailey, Yifei Xu, Guoliang Hu, John Baroch, Yi Zhang, Lanny Pace, Thomas J DeLiberto, Xiu-Feng Wan. A quantitative RT-PCR assay for rapid detection of Eurasian lineage H10 subtype influenza A virus .VIROLOGICA SINICA, 2016, 31(5) : 444-447.  http://dx.doi.org/10.1007/s12250-016-3826-1
    shu

    A quantitative RT-PCR assay for rapid detection of Eurasian lineage H10 subtype influenza A virus

    • 1.

      Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi 39762, USA

    • 2.

      College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China

    • 3.

      Animal and Plant Health Inspection Service of the US Department of Agriculture, Fort Collins 80526, USA

    • 4.

      Mississippi Veterinary Research and Diagnostic Laboratory, College of Veterinary Medicine, Mississippi State University, Mississippi 39762, USA

    • Corresponding author: Xiu-Feng Wan, wan@cvm.msstate.edu, ORCID: 0000-0003-2629-9234
    • Published Date: 18 September 2016
      Available online: 01 October 2016
    • Quantitative RT-PCR (qRT-PCR) has been commonly used for IAV detection in influenza surveillance and disease diagnosis because of its high sensitivity, specificity, and high throughput. A number of HA subtype specific qRT-PCR methods have been developed, including H5, H7, and H9 (Monne et al., 2008). However, there is still lack of a specific qRT-PCR method for detecting the emerging H10N8 IAVs. In this study, a Eurasian-lineage H10 specific qRT-PCR is developed and validated.
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      A quantitative RT-PCR assay for rapid detection of Eurasian lineage H10 subtype influenza A virus

        Corresponding author: Xiu-Feng Wan, wan@cvm.msstate.edu
      • 1. Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi 39762, USA
      • 2. College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China
      • 3. Animal and Plant Health Inspection Service of the US Department of Agriculture, Fort Collins 80526, USA
      • 4. Mississippi Veterinary Research and Diagnostic Laboratory, College of Veterinary Medicine, Mississippi State University, Mississippi 39762, USA
      • Published Date: 18 September 2016

      Abstract: Quantitative RT-PCR (qRT-PCR) has been commonly used for IAV detection in influenza surveillance and disease diagnosis because of its high sensitivity, specificity, and high throughput. A number of HA subtype specific qRT-PCR methods have been developed, including H5, H7, and H9 (Monne et al., 2008). However, there is still lack of a specific qRT-PCR method for detecting the emerging H10N8 IAVs. In this study, a Eurasian-lineage H10 specific qRT-PCR is developed and validated.

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