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Volume 35 Issue 4
August 2020
    Citation: Tian-Jiao Fan, Li Sun, Xian-Guang Yang, Xia Jin, Wei-Wei Sun, Jian-Hua Wang. The Establishment of an In Vivo HIV-1 Infection Model in Humanized B-NSG Mice .VIROLOGICA SINICA, 2020, 35(4) : 417-425.  http://dx.doi.org/10.1007/s12250-019-00181-6
    shu

    The Establishment of an In Vivo HIV-1 Infection Model in Humanized B-NSG Mice

    cstr: 32224.14.s12250-019-00181-6
    • 1.

      CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200031, China

    • 2.

      University of Chinese Academy of Sciences, Beijing 100039, China

    • 3.

      College of Life Science, Henan Normal University, Xinxiang 453007, China

    • Corresponding author: Wei-Wei Sun, wwsun@ips.ac.cn, ORCID: http://orcid.org/0000-0002-4578-6292
      Jian-Hua Wang, jh_wang@sibs.ac.cn, ORCID: http://orcid.org/0000-0002-6435-9907
    • Tian-Jiao Fan and Li Sun have contributed equally to this work.
    • Received Date: 12 July 2019
      Accepted Date: 02 December 2019
      Published Date: 21 December 2019
      Available online: 01 August 2020
    • Suitable animal models for human immunodeficiency virus type 1 (HIV-1) infection are important for elucidating viral pathogenesis and evaluating antiviral strategies in vivo. The B-NSG (NOD-PrkdcscidIl2rgtm1/Bcge) mice that have severe immune defect phenotype are examined for the suitability of such a model in this study. Human peripheral blood mononuclear cells (PBMCs) were engrafted into B-NSG mice via mouse tail vein injection, and the repopulated human T-lymphocytes were observed at as early as 3-weeks post-transplantation in mouse peripheral blood and several tissues. The humanized mice could be infected by HIV-1, and the infection recapitulated features of T-lymphocyte dynamic observed in HIV-1 infected humans, meanwhile the administration of combination antiretroviral therapy (cART) suppressed viral replication and restored T lymphocyte abnormalities. The establishment of HIV-1 infected humanized B-NSG mice not only provides a model to study virus and T cell interplays, but also can be a useful tool to evaluate antiviral strategies.
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      The Establishment of an In Vivo HIV-1 Infection Model in Humanized B-NSG Mice

        Corresponding author: Wei-Wei Sun, wwsun@ips.ac.cn
        Corresponding author: Jian-Hua Wang, jh_wang@sibs.ac.cn
      • 1. CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200031, China
      • 2. University of Chinese Academy of Sciences, Beijing 100039, China
      • 3. College of Life Science, Henan Normal University, Xinxiang 453007, China
      • Received Date: 12 July 2019
      • Accepted Date: 02 December 2019
      • Published Date: 21 December 2019

      Abstract: Suitable animal models for human immunodeficiency virus type 1 (HIV-1) infection are important for elucidating viral pathogenesis and evaluating antiviral strategies in vivo. The B-NSG (NOD-PrkdcscidIl2rgtm1/Bcge) mice that have severe immune defect phenotype are examined for the suitability of such a model in this study. Human peripheral blood mononuclear cells (PBMCs) were engrafted into B-NSG mice via mouse tail vein injection, and the repopulated human T-lymphocytes were observed at as early as 3-weeks post-transplantation in mouse peripheral blood and several tissues. The humanized mice could be infected by HIV-1, and the infection recapitulated features of T-lymphocyte dynamic observed in HIV-1 infected humans, meanwhile the administration of combination antiretroviral therapy (cART) suppressed viral replication and restored T lymphocyte abnormalities. The establishment of HIV-1 infected humanized B-NSG mice not only provides a model to study virus and T cell interplays, but also can be a useful tool to evaluate antiviral strategies.

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