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Volume 36 Issue 3
June 2021
    Citation: Wu-Mei Yuan, Ya-Ge Fan, Meng Cui, Ting Luo, Ya-E Wang, Zhan-Jun Shu, Juan Zhao, Jun Zheng, Yan Zeng. SOX5 Regulates Cell Proliferation, Apoptosis, Migration and Invasion in KSHV-Infected Cells .VIROLOGICA SINICA, 2021, 36(3) : 449-457.  http://dx.doi.org/10.1007/s12250-020-00313-3
    shu

    SOX5 Regulates Cell Proliferation, Apoptosis, Migration and Invasion in KSHV-Infected Cells

    cstr: 32224.14.s12250-020-00313-3
    • 1.

      Department of Stomatology, The First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi 832000, China

    • 2.

      Key Laboratory of Xinjiang Endemic and Ethnic Disease and Department of Biochemistry, School of Medicine, Shihezi University, Shihezi 832000, China

    • 3.

      AIDS Research Office, National Traditional Chinese Medicine Research Base in Xinjiang and the Sixth People's Hospital of Xinjiang Uygur Autonomous Region, Ürümqi 830000, China

    • Corresponding author: Jun Zheng, haohaoay@yeah.net, ORCID: 0000-0002-9998-0832
      Yan Zeng, zengyan910@gmail.com, ORCID: 0000-0002-9823-1266
    • Wu-Mei Yuan and Ya-Ge Fan have contributed equally to this work.
    • Received Date: 01 July 2020
      Accepted Date: 16 September 2020
      Published Date: 24 November 2020
      Available online: 01 June 2021

    • Kaposi's sarcoma (KS) originates from vascular endothelial cells, with KS-associated herpesvirus (KSHV) as the etiological agent. SRY-box transcription factor 5 (SOX5) plays different roles in various types of cancer, although its role in KS remains poorly understood. In this study, we identified the role of SOX5 in KS tissues and KSHV-infected cells and elucidated the molecular mechanism. Thirty-two KS patients were enrolled in this study. Measurement of SOX5 mRNA and protein levels in human KS tissues and adjacent control tissues revealed lower levels in KS tissues, with KS patients having higher SOX5 level in the early stages of the disease compared to the later stages. And SOX5 mRNA and protein was also lower in KSHV-infected cells (iSLK-219 and iSLK-BAC) than normal cells (iSLK-Puro). Additionally, SOX5 overexpression inhibited cell proliferation and promoted apoptosis and decreased KSHV-infected cell migration and invasion. Moreover, we found that SOX5 overexpression suppressed the epithelial-to-mesenchymal transition of KSHV-infected cells. These results suggest SOX5 is a suppressor factor during KS development and a potential target for KS treatment.

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      SOX5 Regulates Cell Proliferation, Apoptosis, Migration and Invasion in KSHV-Infected Cells

        Corresponding author: Jun Zheng, haohaoay@yeah.net
        Corresponding author: Yan Zeng, zengyan910@gmail.com
      • 1. Department of Stomatology, The First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi 832000, China
      • 2. Key Laboratory of Xinjiang Endemic and Ethnic Disease and Department of Biochemistry, School of Medicine, Shihezi University, Shihezi 832000, China
      • 3. AIDS Research Office, National Traditional Chinese Medicine Research Base in Xinjiang and the Sixth People's Hospital of Xinjiang Uygur Autonomous Region, Ürümqi 830000, China
      • Received Date: 01 July 2020
      • Accepted Date: 16 September 2020
      • Published Date: 24 November 2020

      Abstract: 

      Kaposi's sarcoma (KS) originates from vascular endothelial cells, with KS-associated herpesvirus (KSHV) as the etiological agent. SRY-box transcription factor 5 (SOX5) plays different roles in various types of cancer, although its role in KS remains poorly understood. In this study, we identified the role of SOX5 in KS tissues and KSHV-infected cells and elucidated the molecular mechanism. Thirty-two KS patients were enrolled in this study. Measurement of SOX5 mRNA and protein levels in human KS tissues and adjacent control tissues revealed lower levels in KS tissues, with KS patients having higher SOX5 level in the early stages of the disease compared to the later stages. And SOX5 mRNA and protein was also lower in KSHV-infected cells (iSLK-219 and iSLK-BAC) than normal cells (iSLK-Puro). Additionally, SOX5 overexpression inhibited cell proliferation and promoted apoptosis and decreased KSHV-infected cell migration and invasion. Moreover, we found that SOX5 overexpression suppressed the epithelial-to-mesenchymal transition of KSHV-infected cells. These results suggest SOX5 is a suppressor factor during KS development and a potential target for KS treatment.

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