Autophagy induced by human adenovirus B7 structural protein VI inhibits viral replication

Linlin Zhang; Yali Duan; Wei Wang; Qi Li; Jiao Tian; Yun Zhu; Ran Wang; Zhengde Xie

doi:10.1016/j.virs.2023.08.002

October 2023

Citation: Linlin Zhang, Yali Duan, Wei Wang, Qi Li, Jiao Tian, Yun Zhu, Ran Wang, Zhengde Xie. Autophagy induced by human adenovirus B7 structural protein VI inhibits viral replication .VIROLOGICA SINICA, 2023, 38(5) : 709-722. http://dx.doi.org/10.1016/j.virs.2023.08.002

Autophagy induced by human adenovirus B7 structural protein VI inhibits viral replication

Linlin Zhang ^a,b ,
Yali Duan ^a,b,c ,
Wei Wang ^a,b,d ,
Qi Li ^a,b ,
Jiao Tian ^a,b ,
Yun Zhu ^a,b ,
Ran Wang ^a,b ,
Zhengde Xie ^{a,b
,,}

a. Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, 100045, China;
b. Research Unit of Critical Infection in Children, 2019RU016, Chinese Academy of Medical Sciences, Beijing, 100045, China;
c. Department of Infectious Diseases, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, 100045, China;
d. Beijing Coal Group General Hospital, Beijing, 100045, China

Corresponding author: Zhengde Xie, xiezhengde@bch.com.cn
Received Date: 09 February 2023
Accepted Date: 31 July 2023
Available online: 05 August 2023

Abstract

Human adenovirus B7 (HAdV-B7) causes severe acute lower respiratory tract infections in children. However, neither the child-specific antivirals or vaccines are available, nor the pathogenesis is clear. Autophagy, as part of innate immunity, plays an important role in resistance to viral infection by degrading the virus and promoting the development of innate and adaptive immunity. This study provided evidence that HAdV-B7 infection induced complete autophagic flux, and the pharmacological induction of autophagy decreased HAdV-B7 replication. In this process, the host protein Bcl2-associated athanogene 3 (BAG3) mediated autophagy to inhibit the replication of HAdV-B7 by binding to the PPSY structural domain of viral protein pVI through its WW structural domain. These findings further our understanding of the host immune response during viral infection and will help to develop broad anti-HAdV therapies.
- Human adenovirus B7 (HAdV-B7)
- , Autophagy
- , Bcl2-associated athanogene 3 (BAG3)
- , Virus replication

Electronic Supplementary Material

10.1016j.virs.2023.08.002-ESM3.xlsx
10.1016j.virs.2023.08.002-ESM2.xlsx
10.1016j.virs.2023.08.002-ESM1.docx

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Autophagy induced by human adenovirus B7 structural protein VI inhibits viral replication

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Corresponding author: Zhengde Xie, xiezhengde@bch.com.cn

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