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Volume 38 Issue 5
October 2023
    Citation: Lijuan Lu, Yuanyun Ao, Ran Jia, Huaqing Zhong, Pengcheng Liu, Menghua Xu, Liyun Su, Lingfeng Cao, Jin Xu. Changing predominance of norovirus strains in children with acute gastroenteritis in Shanghai, 2018–2021 .VIROLOGICA SINICA, 2023, 38(5) : 671-679.  http://dx.doi.org/10.1016/j.virs.2023.08.005
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    Changing predominance of norovirus strains in children with acute gastroenteritis in Shanghai, 2018–2021

    • a. Department of Clinical Laboratory, Children's Hospital of Fudan University, National Children's Medical Center, Shanghai, 201100, China;

    • b. Shanghai Institute of Infectious Disease and Biosecurity, Fudan University, Shanghai, 201100, China

    • Corresponding author: Jin Xu, jinxu_125@163.com
    • Received Date: 14 April 2023
      Accepted Date: 17 August 2023
      Available online: 22 August 2023
    • Norovirus (NoV) is a major pathogen that causes acute gastroenteritis (AGE) in people of all ages, especially in children. In this study, we investigated the molecular epidemiological characteristics of NoV in children with AGE in Shanghai from 2018 to 2021. The overall detection rate of NoV was 11.9% (181/1545), with annual detection rates of 9.4% (36/381), 13.6% (29/213), 5.8% (13/226) and 14.2% (103/725), respectively. Of note, the prevalence of NoV in 2020 was significantly lower than that in 2018-2019 (10.9%, 65/594) (P=0.023) and 2021 (14.2%, 103/725) (P=0.000). The 181 NoV strains identified in this study were classified into the GI group (1.1%, 2/181), GII group (98.3%, 178/181) and GIX group (0.6%, 1/181) according to the VP1 gene. The most common NoV VP1 genotype was GII.4 Sydney_2012 (63.5%, 115/181), followed by GII.3 (19.9%, 36/181) and GII.2 (9.4%, 17/181). For P genotypes, 174 strains were sequenced successfully according to the RdRp gene, and the predominant genotype was GII.P16 (44.8%, 78/174), followed by GII.P31 (25.9%, 45/174) and GII.P12 (21.3%, 37/174). Among the 174 cases, GII.4 Sydney_2012[P16] (36.8%, 64/174) was the dominant genotype, followed by GII.4 Sydney_2012[P31] (25.3%, 44/174), GII.3[P12] (20.1%, 35/174) and GII.2[P16] (8.0%, 14/174). In particular, the dominant genotypes in Shanghai changed from GII.4 Sydney_2012[P31] in 2018-2019 to GII.4 Sydney_2012[P16] in 2020-2021. This is the first report to describe the epidemiological changes in NoV infection before and during the COVID-19 pandemic in Shanghai. These data highlight the importance of continuous surveillance for NoV in children with AGE in Shanghai.
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      10.1016j.virs.2023.08.005-ESM.rtf

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      Changing predominance of norovirus strains in children with acute gastroenteritis in Shanghai, 2018–2021

        Corresponding author: Jin Xu, jinxu_125@163.com
      • a. Department of Clinical Laboratory, Children's Hospital of Fudan University, National Children's Medical Center, Shanghai, 201100, China;
      • b. Shanghai Institute of Infectious Disease and Biosecurity, Fudan University, Shanghai, 201100, China
      • Received Date: 14 April 2023
      • Accepted Date: 17 August 2023

      Abstract: Norovirus (NoV) is a major pathogen that causes acute gastroenteritis (AGE) in people of all ages, especially in children. In this study, we investigated the molecular epidemiological characteristics of NoV in children with AGE in Shanghai from 2018 to 2021. The overall detection rate of NoV was 11.9% (181/1545), with annual detection rates of 9.4% (36/381), 13.6% (29/213), 5.8% (13/226) and 14.2% (103/725), respectively. Of note, the prevalence of NoV in 2020 was significantly lower than that in 2018-2019 (10.9%, 65/594) (P=0.023) and 2021 (14.2%, 103/725) (P=0.000). The 181 NoV strains identified in this study were classified into the GI group (1.1%, 2/181), GII group (98.3%, 178/181) and GIX group (0.6%, 1/181) according to the VP1 gene. The most common NoV VP1 genotype was GII.4 Sydney_2012 (63.5%, 115/181), followed by GII.3 (19.9%, 36/181) and GII.2 (9.4%, 17/181). For P genotypes, 174 strains were sequenced successfully according to the RdRp gene, and the predominant genotype was GII.P16 (44.8%, 78/174), followed by GII.P31 (25.9%, 45/174) and GII.P12 (21.3%, 37/174). Among the 174 cases, GII.4 Sydney_2012[P16] (36.8%, 64/174) was the dominant genotype, followed by GII.4 Sydney_2012[P31] (25.3%, 44/174), GII.3[P12] (20.1%, 35/174) and GII.2[P16] (8.0%, 14/174). In particular, the dominant genotypes in Shanghai changed from GII.4 Sydney_2012[P31] in 2018-2019 to GII.4 Sydney_2012[P16] in 2020-2021. This is the first report to describe the epidemiological changes in NoV infection before and during the COVID-19 pandemic in Shanghai. These data highlight the importance of continuous surveillance for NoV in children with AGE in Shanghai.

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