Citation: Yabin Hu, Jun Chen, Jing Yang, Zhanpeng Liu, Xiaoyue Zhang, Qian Wu, Lulu Liu, Shishan Teng, Rongzhang He, Bo Liu, Xingyu Zheng, Rui Lu, Dong Pan, You Wang, Liting Peng, Hongying chen, Yi-Ping Li, Wenpei Liu, Xiaowang Qu. Isolation and characterization of spike S2-specific monoclonal antibodies with reactivity to pan-coronaviruses .VIROLOGICA SINICA, 2024, 39(1) : 169-172.  http://dx.doi.org/10.1016/j.virs.2023.10.008

Isolation and characterization of spike S2-specific monoclonal antibodies with reactivity to pan-coronaviruses

  • Highlights
    1. Five broad-spectrum mAbs identified from COVID-19 convalescents and vaccinees possessed the ability to recognize spike S2 of pan-coronaviruses.
    2. Five mAbs targeted distinct but conserved epitopes on spike S2 of pan-coronaviruses.
    3. Three of these five mAbs were competitively bound to the fusion peptide epitopes of coronavirus spike protein.

  • 加载中
  • 10.1016j.virs.2023.10.008-ESM.docx
    1. Andreano E, Nicastri E, Paciello I, Pileri P, Manganaro N, Piccini G, Manenti A, Pantano E, Kabanova A, Troisi M, Vacca F, Cardamone D, De Santi C, Torres JL, Ozorowski G, Benincasa L, Jang H, Di Genova C, Depau L, Brunetti J, Agrati C, Capobianchi MR, Castilletti C, Emiliozzi A, Fabbiani M, Montagnani F, Bracci L, Sautto G, Ross TM, Montomoli E, Temperton N, Ward AB, Sala C, Ippolito G, Rappuoli R. 2021. Extremely potent human monoclonal antibodies from covid-19 convalescent patients. Cell, 184: 1821-1835 e1816.

    2. Chen J, Yang J, Chang F, Hu Y, Wu Q, Teng S, Liu Y, Zhang J, He R, Liu B, Zheng X, Liu Z, Peng Y, Xie Z, Zhang Y, Lu R, Pan D, Wang Y, Peng L, Liu W, Li YP, Qu X. 2023. Identification of broad neutralizing antibodies against omicron subvariants from covid-19 convalescents and vaccine recipients. Virol Sin, 38: 313-316.

    3. Ge XY, Li JL, Yang XL, Chmura AA, Zhu G, Epstein JH, Mazet JK, Hu B, Zhang W, Peng C, Zhang YJ, Luo CM, Tan B, Wang N, Zhu Y, Crameri G, Zhang SY, Wang LF, Daszak P, Shi ZL. 2013. Isolation and characterization of a bat sars-like coronavirus that uses the ace2 receptor. Nature, 503: 535-538.

    4. Klompus S, Leviatan S, Vogl T, Mazor RD, Kalka IN, Stoler-Barak L, Nathan N, Peres A, Moss L, Godneva A, Kagan Ben Tikva S, Shinar E, Dvashi HC, Gabizon R, London N, Diskin R, Yaari G, Weinberger A, Shulman Z, Segal E. 2021. Cross-reactive antibodies against human coronaviruses and the animal coronavirome suggest diagnostics for future zoonotic spillovers. Sci Immunol, 6.

    5. Kreer C, Zehner M, Weber T, Ercanoglu MS, Gieselmann L, Rohde C, Halwe S, Korenkov M, Schommers P, Vanshylla K, Di Cristanziano V, Janicki H, Brinker R, Ashurov A, Krahling V, Kupke A, Cohen-Dvashi H, Koch M, Eckert JM, Lederer S, Pfeifer N, Wolf T, Vehreschild M, Wendtner C, Diskin R, Gruell H, Becker S, Klein F. 2020. Longitudinal isolation of potent near-germline sars-cov-2-neutralizing antibodies from covid-19 patients. Cell, 182: 843-854 e812.

    6. Meekins DA, Morozov I, Trujillo JD, Gaudreault NN, Bold D, Carossino M, Artiaga BL, Indran SV, Kwon T, Balaraman V, Madden DW, Feldmann H, Henningson J, Ma W, Balasuriya UBR, Richt JA. 2020. Susceptibility of swine cells and domestic pigs to sars-cov-2. Emerg Microbes Infect, 9: 2278-2288.

    7. Ng KW, Faulkner N, Finsterbusch K, Wu M, Harvey R, Hussain S, Greco M, Liu Y, Kjaer S, Swanton C, Gandhi S, Beale R, Gamblin SJ, Cherepanov P, McCauley J, Daniels R, Howell M, Arase H, Wack A, Bauer DLV, Kassiotis G. 2022. Sars-cov-2 s2-targeted vaccination elicits broadly neutralizing antibodies. Sci Transl Med, 14: eabn3715.

    8. Shi J, Wen Z, Zhong G, Yang H, Wang C, Huang B, Liu R, He X, Shuai L, Sun Z, Zhao Y, Liu P, Liang L, Cui P, Wang J, Zhang X, Guan Y, Tan W, Wu G, Chen H, Bu Z. 2020. Susceptibility of ferrets, cats, dogs, and other domesticated animals to sars-coronavirus 2. Science, 368: 1016-1020.

    9. Sun X, Yi C, Zhu Y, Ding L, Xia S, Chen X, Liu M, Gu C, Lu X, Fu Y, Chen S, Zhang T, Zhang Y, Yang Z, Ma L, Gu W, Hu G, Du S, Yan R, Fu W, Yuan S, Qiu C, Zhao C, Zhang X, He Y, Qu A, Zhou X, Li X, Wong G, Deng Q, Zhou Q, Lu H, Ling Z, Ding J, Lu L, Xu J, Xie Y, Sun B. 2022. Neutralization mechanism of a human antibody with pan-coronavirus reactivity including sars-cov-2. Nat Microbiol, 7: 1063-1074.

    10. Tan W, Zhao X, Ma X, Wang W, Niu P, Xu W, Gao GF, Wu G. 2020. A novel coronavirus genome identified in a cluster of pneumonia cases - wuhan, china 2019-2020. China CDC Wkly, 2: 61-62.

    11. Wang N, Li SY, Yang XL, Huang HM, Zhang YJ, Guo H, Luo CM, Miller M, Zhu G, Chmura AA, Hagan E, Zhou JH, Zhang YZ, Wang LF, Daszak P, Shi ZL. 2018. Serological evidence of bat sars-related coronavirus infection in humans, china. Virol Sin, 33: 104-107.

    12. Xiao K, Zhai J, Feng Y, Zhou N, Zhang X, Zou JJ, Li N, Guo Y, Li X, Shen X, Zhang Z, Shu F, Huang W, Li Y, Zhang Z, Chen RA, Wu YJ, Peng SM, Huang M, Xie WJ, Cai QH, Hou FH, Chen W, Xiao L, Shen Y. 2020. Isolation of sars-cov-2-related coronavirus from malayan pangolins. Nature, 583: 286-289.

    13. Xu Z, Kang X, Han P, Du P, Li L, Zheng A, Deng C, Qi J, Zhao X, Wang Q, Liu K, Gao GF. 2022. Binding and structural basis of equine ace2 to rbds from sars-cov, sars-cov-2 and related coronaviruses. Nat Commun, 13: 3547.

    14. Zhu N, Zhang D, Wang W, Li X, Yang B, Song J, Zhao X, Huang B, Shi W, Lu R, Niu P, Zhan F, Ma X, Wang D, Xu W, Wu G, Gao GF, Tan W, China Novel Coronavirus I, Research T. 2020. A novel coronavirus from patients with pneumonia in china, 2019. N Engl J Med, 382: 727-733.

    15. Zost SJ, Gilchuk P, Chen RE, Case JB, Reidy JX, Trivette A, Nargi RS, Sutton RE, Suryadevara N, Chen EC, Binshtein E, Shrihari S, Ostrowski M, Chu HY, Didier JE, MacRenaris KW, Jones T, Day S, Myers L, Eun-Hyung Lee F, Nguyen DC, Sanz I, Martinez DR, Rothlauf PW, Bloyet LM, Whelan SPJ, Baric RS, Thackray LB, Diamond MS, Carnahan RH, Crowe JE, Jr. 2020. Rapid isolation and profiling of a diverse panel of human monoclonal antibodies targeting the sars-cov-2 spike protein. Nat Med, 26: 1422-1427.

  • 加载中

Article Metrics

Article views(1466) PDF downloads(16) Cited by()

Related
Proportional views

    Isolation and characterization of spike S2-specific monoclonal antibodies with reactivity to pan-coronaviruses

      Corresponding author: Hongying chen, chenhy@nwsuaf.edu.cn
      Corresponding author: Yi-Ping Li, lyiping@mail.sysu.edu.cn
      Corresponding author: Wenpei Liu, wenpeiliu_2008@foxmail.com
      Corresponding author: Xiaowang Qu, quxiaowang@163.com
    • a. College of Basic Medical Sciences, Hengyang Medical School, University of South China & MOE Key Lab of Rare Pediatric Diseases, Hengyang, 421001, China;
    • b. Translational Medicine Institute, The First People's Hospital of Chenzhou, Hengyang Medical School, University of South China, Chenzhou, 423000, China;
    • c. College of Life Sciences, Northwest A&F University, Yangling, 712100, China;
    • d. Institute of Human Virology, Department of Pathogen Biology and Biosecurity, and Key Laboratory of Tropical Disease Control of Ministry of Education, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China;
    • e. School of Public Health, University of South China, Hengyang, 421001, China

    Abstract: Highlights
    1. Five broad-spectrum mAbs identified from COVID-19 convalescents and vaccinees possessed the ability to recognize spike S2 of pan-coronaviruses.
    2. Five mAbs targeted distinct but conserved epitopes on spike S2 of pan-coronaviruses.
    3. Three of these five mAbs were competitively bound to the fusion peptide epitopes of coronavirus spike protein.

    Reference (15) Relative (19)

    目录

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return