Phylogenitc analysis and immunogenicity comparison of porcine genotype G9 rotavirus in China from 2020-2023

Yudi Pan; Zixin Li; Qian Miao; Hongyan Shi; Longjun Guo; Li Feng; Jin Tian

doi:10.1016/j.virs.2025.02.003

April 2025

Citation: Yudi Pan, Zixin Li, Qian Miao, Hongyan Shi, Longjun Guo, Li Feng, Jin Tian. Phylogenitc analysis and immunogenicity comparison of porcine genotype G9 rotavirus in China from 2020-2023 .VIROLOGICA SINICA, 2025, 40(2) : 176-185. http://dx.doi.org/10.1016/j.virs.2025.02.003

Phylogenitc analysis and immunogenicity comparison of porcine genotype G9 rotavirus in China from 2020-2023

State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China

Corresponding author: Li Feng, fengli@caas.cn
Jin Tian, tj6049345@126.com
Received Date: 30 September 2024
Accepted Date: 12 February 2025
Available online: 21 February 2025

Abstract

As an emerging genotype, the G9 genotype rotaviruses (RVs) are widespread among humans and pigs, and have been reported in many countries and regions in the recent years. Moreover, porcine G9 strains could cross the interspecies barrier to infect human. To investigate the epidemic trends of porcine G9 strains as well as the cross-immunoreactivity among different isolates, an epidemiological investigation about porcine G9 genotype RVs (PoRVs) was performed during the period 2020-2023 in multiple provinces of China. A total of nine representative strains were identified. The phylogenetic analysis based on viral VP7 gene showed that these strains mainly clustered with lineages III and VI, which revealed the predominant G9 PoRVs in China. Moreover, a new lineage, lineage VII, was identified, and strains of this lineage were found to be circulating in Guangdong and Taiwan. Except lineages I and IV, some isolates from other lineages could co-circulate in pigs and humans. Three G9 strains, namely 923H, 923E, and 923X, which belonged to the largest sub-lineage III, were isolated. Then, the significant cross-reactivity was observed among strains of the same or different lineages. This study is the first to systematically investigate the genetic and immunogenetic characteristics of porcine G9 genotype rotavirus in China, as well as the potential cross-species transmission between pigs and humans, providing a valuable direction for the effective prevention of porcine rotavirus.
- Rotavirus (RV)
- , G9
- , Pig
- , Genetic and immunogenetic characterization

Electronic Supplementary Material

10.1016j.virs.2025.02.003-ESM.docx
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