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Volume 22 Issue 2
April 2007
    Citation: Christopher J. Lucarotti, Benoit Morin, Robert I. Graham, Renée Lapointe. Production, Application, and Field Performance of AbietivTM, the Balsam Fir Sawfly Nucleopolyhedrovirus .VIROLOGICA SINICA, 2007, 22(2) : 163-172.
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    Production, Application, and Field Performance of AbietivTM, the Balsam Fir Sawfly Nucleopolyhedrovirus

    • 1.

      Natural Resources Canada, Canadian Forest Service -Atlantic Forestry Centre, PO Box 4000, Fredericton, New Brunswick E3B 5P7, Canada

    • 2.

      Population Ecology Group, Faculty of Forestry and Environmental Management, University of New Brunswick, Fredericton, New Brunswick E3B 6C2, Canada

    • 3.

      Sylvar Technologies Inc., P.O. Box 636, Stn. A, Fredericton, New Brunswick E3B 5A6, Canada

    • Corresponding author: Christopher J. Lucarotti, clucarot@nrcan.gc.ca
    • Received Date: 08 February 2007
      Accepted Date: 12 February 2007
      Available online: 01 April 2007
    • Beginning in the early 1990s, the balsam fir sawfly (Neodiprion abietis) became a significant defoliating insect of precommercially thinned balsam fir (Abies balsamea (L.) Mill.) stands in western Newfoundland, Canada. In 1997, a nucleopolyhedrovirus (NeabNPV) was isolated from the balsam fir sawfly and, as no control measures were then available, NeabNPV was developed for the biological control of balsam fir sawfly. In order to register NeabNPV for operational use under the Canadian Pest Control Products Act, research was carried out in a number of areas including NeabNPV field efficacy, non-target organism toxicology, balsam fir sawfly ecology and impact on balsam fir trees, and NeabNPV genome sequencing and analysis. As part of the field efficacy trials, approximately 22 500 hectares of balsam fir sawfly-infested forest were aerially treated with NeabNPV between 2000 and 2005. NeabNPV was found to be safe, efficacious, and economical for the suppression of balsam fir sawfly outbreak populations. Conditional registration for the NeabNPV-based product, Abietiv, was received from the Pest Management Regulatory Agency (Health Canada) in April 2006. In July 2006, Abietiv was applied by spray airplanes to 15 000 ha of balsam fir sawfly-infested forest in western Newfoundland in an operational control program.
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      16. Hawtin R E, Zarkowska T, Arnold K, et al. 1997.Liquefaction of Autographa californica nucleopolyhedrovirus-infected insects is dependent on the integrity of virus-encoded chitinase and cathepsin genes. Virology, 238: 243-253.
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      21. Lauzon H A M, Garcia-Maruniak A, Zanotto P M A, et al. 2006. Genomic comparison of Neodiprion sertifer and Neodiprion lecontei nucleopolyhedroviruses and identification of potential hymenopteran baculovirus specific ORFs. J Gen Virol, 87: 1491-1500.
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      22. Lauzon H A M, Lucarotti C J, Krell P J, et al. 2004.Sequence and organization of the Neodiprion lecontei nucleopolyhedrovirus genome. J Virol, 78: 7023-7035.
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      23. Little C H A, Lavigne M B, Ostaff D P. 2003. Impact of old foliage removal, simulating defoliation by the balsam fir sawfly, on balsam fir tree growth and photosynthesis of current-year shoots. For Ecol Manage, 186: 261-269.
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      24. Lucarotti C J, Kettela E G, Mudryj G. 2006. The registration of AbietivTM: a biological control product based on Neodiprion abietis nucleopolyhedrovirus for use against its natural host, the balsam fir sawfly. SERG International Report. p 47.

      25. Lucarotti C J, Moreau G, Kettela E G. 2007. AbietivTM-a biological product for the control of the balsam fir sawfly. In: Biological Control: International Case Studies (Vincent C, Goettel M, Lazrovits G. eds. ). CABI Publishing, UK. In press.

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      Production, Application, and Field Performance of AbietivTM, the Balsam Fir Sawfly Nucleopolyhedrovirus

        Corresponding author: Christopher J. Lucarotti, clucarot@nrcan.gc.ca
      • 1. Natural Resources Canada, Canadian Forest Service -Atlantic Forestry Centre, PO Box 4000, Fredericton, New Brunswick E3B 5P7, Canada
      • 2. Population Ecology Group, Faculty of Forestry and Environmental Management, University of New Brunswick, Fredericton, New Brunswick E3B 6C2, Canada
      • 3. Sylvar Technologies Inc., P.O. Box 636, Stn. A, Fredericton, New Brunswick E3B 5A6, Canada
      • Received Date: 08 February 2007
      • Accepted Date: 12 February 2007

      Abstract: Beginning in the early 1990s, the balsam fir sawfly (Neodiprion abietis) became a significant defoliating insect of precommercially thinned balsam fir (Abies balsamea (L.) Mill.) stands in western Newfoundland, Canada. In 1997, a nucleopolyhedrovirus (NeabNPV) was isolated from the balsam fir sawfly and, as no control measures were then available, NeabNPV was developed for the biological control of balsam fir sawfly. In order to register NeabNPV for operational use under the Canadian Pest Control Products Act, research was carried out in a number of areas including NeabNPV field efficacy, non-target organism toxicology, balsam fir sawfly ecology and impact on balsam fir trees, and NeabNPV genome sequencing and analysis. As part of the field efficacy trials, approximately 22 500 hectares of balsam fir sawfly-infested forest were aerially treated with NeabNPV between 2000 and 2005. NeabNPV was found to be safe, efficacious, and economical for the suppression of balsam fir sawfly outbreak populations. Conditional registration for the NeabNPV-based product, Abietiv, was received from the Pest Management Regulatory Agency (Health Canada) in April 2006. In July 2006, Abietiv was applied by spray airplanes to 15 000 ha of balsam fir sawfly-infested forest in western Newfoundland in an operational control program.

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