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Volume 04 Issue 1
February 1989
    Citation: TU Rong-Han, TAN Fu-Lai, CHEN Hong-Kai, SU Yuan, TAO Yi-Xin, NI Da-Dan, LIAO De-Fu. Preparation and Application of the Monoclonal Hybridoma Antibodies against the Epidemic Hemorrhagic Fever Virus (EHFV) in Anhui Province .VIROLOGICA SINICA, 1989, 4(1) : 28.
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    Preparation and Application of the Monoclonal Hybridoma Antibodies against the Epidemic Hemorrhagic Fever Virus (EHFV) in Anhui Province

    • 1.

      Division of Virclogy

    • 2.

      Anhui Institute of Medical Sciences

    • Available online: 05 March 1989
    • Two monoclonal antibodies(McAb) were obtained by fusion of SP2/O myeloma cells with spleen cells from Balb/c mice. They were respectively immunized with the Li strain and the hemagglutinin(HAN) from the Chen strain of EHFV. Two fusions had been done. The growth rate of hybridoma was 97.5% and 60%. The positive rate was 2.5% and 22%. The positive rate of cloning all achieved 100%. Finally two hybridoma cell lines had been established. They were separately designated as 2B_7 and 1H_8. The titers of two ascite...
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      Preparation and Application of the Monoclonal Hybridoma Antibodies against the Epidemic Hemorrhagic Fever Virus (EHFV) in Anhui Province

      • 1. Division of Virclogy
      • 2. Anhui Institute of Medical Sciences

      Abstract: Two monoclonal antibodies(McAb) were obtained by fusion of SP2/O myeloma cells with spleen cells from Balb/c mice. They were respectively immunized with the Li strain and the hemagglutinin(HAN) from the Chen strain of EHFV. Two fusions had been done. The growth rate of hybridoma was 97.5% and 60%. The positive rate was 2.5% and 22%. The positive rate of cloning all achieved 100%. Finally two hybridoma cell lines had been established. They were separately designated as 2B_7 and 1H_8. The titers of two ascite...

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