Cloning and ldentification of the Type-specific DNA Fragment of Herpes Simplex Virus Type 2
Abstract: The DNA of Herpes Simplex Virus type 2 (HSV-2)digested with restrictionenzyme BamHI and the BamHI G fragment was isolated, which locates in thereverse repeat sequence and spans the junction site of L and S component.Then the BamHI G was inserted on the BamHI cut-site of the vector plasmidpUC 8. In order to modify this recombinant plasmid, the EcoRI--KPN I fra-gment of the recombinant plasmid was removed by restriction enzyme digestionwith EcoRI and KPN 1. After modification of the ends and ligation of them,...