Serving the Society Since 1986
Advanced Search
Volume 09 Issue 2
April 1994
    Citation: CHEN Xiao-Xu, HOU Chu-Xia, LI Re-Lun. Preparation of Biotin HBV RNA Probe and Its Application in Serum Detection .VIROLOGICA SINICA, 1994, 9(2) : 95-99.
    shu

    Preparation of Biotin HBV RNA Probe and Its Application in Serum Detection

    • 1.

      Department of Microbiology,Military Animal Medicure Cottege of Shen Yanq Comunend，Da Lian 116013

    • Available online: 05 June 1994
    • epotits B virus (HBV)RNA probes were synthesized in vitro by transcription from recombinantsbetween HBV DNA and the transcriptfon vector SP(65).These probes can be labelled with biotin and behybridized spoifically with HBV DNA.Comparing with HBV DNA probe prepared by Nick-translation for detection of HBV DNA,HBVRNA probe are 10 times sensitive than that of HBV DNA.In addition,70 serum saniples of hepatitis B potients have been detected by hybridization withtwo probes.Pooitive proportion of HBV DNA corresponils to 31.42%, 28.57%(P0.25).The clinical application of HBV RNA probes was discusseil preluninarily.
    • 加载中
    • 加载中
    PDF

    Article Metrics

    Article views(4268) PDF downloads(1014) Cited by()

    Related
    Proportional views

      Preparation of Biotin HBV RNA Probe and Its Application in Serum Detection

      • 1. Department of Microbiology,Military Animal Medicure Cottege of Shen Yanq Comunend，Da Lian 116013

      Abstract: epotits B virus (HBV)RNA probes were synthesized in vitro by transcription from recombinantsbetween HBV DNA and the transcriptfon vector SP(65).These probes can be labelled with biotin and behybridized spoifically with HBV DNA.Comparing with HBV DNA probe prepared by Nick-translation for detection of HBV DNA,HBVRNA probe are 10 times sensitive than that of HBV DNA.In addition,70 serum saniples of hepatitis B potients have been detected by hybridization withtwo probes.Pooitive proportion of HBV DNA corresponils to 31.42%, 28.57%(P0.25).The clinical application of HBV RNA probes was discusseil preluninarily.

        HTML

      Relative (20)

      目录

      This journal is a member of, and subscribes to the principles of, the Committee on Publication Ethics (COPE)

      鄂ICP备05001977号

         

      鄂公网安备 42010602003674号

      Copyright © 2019 Virologica Sinica. All rights reserved

      /

      DownLoad:  Full-Size Img  PowerPoint
      Return
      Return