Detection of pseudorabies virus by biotinylated DNA probe

dot blot hybridization method was developed to detect pseudorbies virus DNA in infectedcultured cells.Seven strains of pseudorabies virus were detected by hiotinylated pseudorabies virusFa DNA probe,and recomhinant plasmid pPB11DNA(BamHI-11 fragment of PRV Fa was clonedbetween the BamHI sites of plasmid pBR322)probe.PRV Fa DNA probe could detect 10 pg nu-cleic acid in the samples. The nucleic acid hybridization method is sensitive and specific for diag-nosing pseudorabies virus.