Citation: HAN You-Qi, HUANG Xiang-Rui, ZHANG Min-Li, CHEN Tian-Mi, LIU Yu-Jing. Study on M echanisms of Hepatitis A Virus Inactivation by Ultraviolet Irradiation .VIROLOGICA SINICA, 1997, 12(1) : 83.

Study on M echanisms of Hepatitis A Virus Inactivation by Ultraviolet Irradiation

  • Available online: 05 March 1997
  • This study showed that 300 W/cm2 UV irradiation for 5 min could inactivate 94.63% ofHAV. No obvious changes were found experimentally under this irradiation dose, other than the decrease of RNA transfection ability. After 20 min UV irradiation, all of HAV were inactivated. For the RNA sedimentation, the original single UV absorption peak (32.9S) became two (11 S and 15.2S) after the irradiation. The fragment of 5'-untranslated region (HAV RNA 222~ 819) could not be detected with RT-PCR. The RNA transfection became negative. But the fragment of P1 region (HAV RNA 2823~3049) could still be detected with RT-PCR, the hybridhation shadow of 3'-terminal poly(A) tail with oligo(dT) probe labelled with -(32)P also became slightly lighter, and no obvious changes were found in the antigenicity of HAV detectedwith ELISA and in 4 capsid polypeptides which was separated by 15% SDS-polyacrylamide gel containing 4 mol/L urea.

  • 加载中
  • 加载中

Article Metrics

Article views(3916) PDF downloads(966) Cited by()

Related
Proportional views

    Study on M echanisms of Hepatitis A Virus Inactivation by Ultraviolet Irradiation

    • 1. Institute of Microbiology and Epedemiology,Academy of Military Medica1.Sciences Institute of Biotechnology,Academy of Military Medlcal Sciences.Beijing 1O0850

    Abstract: This study showed that 300 W/cm2 UV irradiation for 5 min could inactivate 94.63% ofHAV. No obvious changes were found experimentally under this irradiation dose, other than the decrease of RNA transfection ability. After 20 min UV irradiation, all of HAV were inactivated. For the RNA sedimentation, the original single UV absorption peak (32.9S) became two (11 S and 15.2S) after the irradiation. The fragment of 5'-untranslated region (HAV RNA 222~ 819) could not be detected with RT-PCR. The RNA transfection became negative. But the fragment of P1 region (HAV RNA 2823~3049) could still be detected with RT-PCR, the hybridhation shadow of 3'-terminal poly(A) tail with oligo(dT) probe labelled with -(32)P also became slightly lighter, and no obvious changes were found in the antigenicity of HAV detectedwith ELISA and in 4 capsid polypeptides which was separated by 15% SDS-polyacrylamide gel containing 4 mol/L urea.

    Relative (20)

    目录

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return