Citation: FANG Fang, PAN Wei, CI Zhong-Tian, SONG Yan-Bin, ZHU Shi-Ying. Sequence characterization of hypervariable region 1 of the putative envelope protein 2 in Chinese HCV isolates .VIROLOGICA SINICA, 1998, 13(1) : 39.

Sequence characterization of hypervariable region 1 of the putative envelope protein 2 in Chinese HCV isolates

  • Available online: 05 March 1998
  • Twenty three serum samples from blood donor, hemodialysis and hepatitis patients were collected. Using reverse transcription nested polymerase chain reaction method (RT nested PCR),the cDNA fragments of the putative envelope protein 2 (E 2/NS 1) of HCV RNA were amplified and then sequenced. Nucleotied and amino acid sequence analysis showed marked heterogeneity among isolates from 23 patients at the N terminus of E 2/NS 1.The extended sequence analysis comfirmed that the hypervariable region one (HVR 1) is located at the positions 1459~1559 in nucleotide sequence,and 384~410 in amino acid sequence. It was found that 15 amino acids of 27 amino acids of HVR 1 in Chinese HCV isolates were stable, and the composition and distribution of amino acid of HVR 1 were much different from 166 isolates reported by Sekiya. It is suggested that studying the characterization of HVR 1 HCV Chinese isolates would be helpful for investigating geographical distribution of HCV, and researching diagnosis method and vacc.

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    Sequence characterization of hypervariable region 1 of the putative envelope protein 2 in Chinese HCV isolates

    • 1. Sequence Characterization of Hypervariable Region 1 of the Putative Envelope Protein 2 in Chinese HCV Isolates

    Abstract: Twenty three serum samples from blood donor, hemodialysis and hepatitis patients were collected. Using reverse transcription nested polymerase chain reaction method (RT nested PCR),the cDNA fragments of the putative envelope protein 2 (E 2/NS 1) of HCV RNA were amplified and then sequenced. Nucleotied and amino acid sequence analysis showed marked heterogeneity among isolates from 23 patients at the N terminus of E 2/NS 1.The extended sequence analysis comfirmed that the hypervariable region one (HVR 1) is located at the positions 1459~1559 in nucleotide sequence,and 384~410 in amino acid sequence. It was found that 15 amino acids of 27 amino acids of HVR 1 in Chinese HCV isolates were stable, and the composition and distribution of amino acid of HVR 1 were much different from 166 isolates reported by Sekiya. It is suggested that studying the characterization of HVR 1 HCV Chinese isolates would be helpful for investigating geographical distribution of HCV, and researching diagnosis method and vacc.

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