Citation: ZHANG Xi-Bing, WANG Tie-Hui, LI Ge-Jiang, GU Fang-Jun, SHU Xiao-Mu. Induction and Partial CharacterjzatiOn Of CAB Fish Cell (Blastulae Embryonic Cell Line of Crueian Carp)Interferon .VIROLOGICA SINICA, 2000, 15(2) : 163-169.

Induction and Partial CharacterjzatiOn Of CAB Fish Cell (Blastulae Embryonic Cell Line of Crueian Carp)Interferon

  • Available online: 05 June 2000
  • The Antiviral substance was induced from blastulae embryonic cell line of crucian carp (CAB) by ultra violet inactivated grass carp reovirus (GCRVuv). This substance was sensitive to trypsin and stable at 56 ℃ pH 211. The activity of this substance was influenced by target cell density, incubation temperature and time with CAB cells, but not decreased by treatment of the special antibody of GCRV. This substance couldnt kill virus directly, but showed anti GCRV and anti STIV activity in various cultured fish cells. Inhibition of viral replication in cells was dependent on the process of cellular RNA transcription and protein synthesis. These characteristics consist with IFN alpha/beta of mammals, and the substance is a kind of crucian carp interferon.

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    Induction and Partial CharacterjzatiOn Of CAB Fish Cell (Blastulae Embryonic Cell Line of Crueian Carp)Interferon

    • 1. Institute of Hydrobiology,Chinese Academy of Sciences, Wuhan 430072,China

    Abstract: The Antiviral substance was induced from blastulae embryonic cell line of crucian carp (CAB) by ultra violet inactivated grass carp reovirus (GCRVuv). This substance was sensitive to trypsin and stable at 56 ℃ pH 211. The activity of this substance was influenced by target cell density, incubation temperature and time with CAB cells, but not decreased by treatment of the special antibody of GCRV. This substance couldnt kill virus directly, but showed anti GCRV and anti STIV activity in various cultured fish cells. Inhibition of viral replication in cells was dependent on the process of cellular RNA transcription and protein synthesis. These characteristics consist with IFN alpha/beta of mammals, and the substance is a kind of crucian carp interferon.

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