Citation: LU ng—mei, CHEN Er-jla, XIE Tian-hor~, ZHUANG Jun—ying.LIU Yong LI Chun—hong, SUN Mao-sheng”, DAI Chang—bai, . DNA Immunization of Hepatitis E Virus(HEV) .VIROLOGICA SINICA, 2001, 16(2) : 131-134.

DNA Immunization of Hepatitis E Virus(HEV)

  • Available online: 05 June 2001
  • Two plasmid constructs,pcE2 and peE3,containing 3’fragment of open reading frame 2 (ORF2,1163 bp)of hepatitis E virus(HEV)and full—length ORF3(369 bp).were injected into bi— lateral tibiaLs of Swiss mice respectively.for three times(0,2 and 4 weeks)and observed the HEv IgG by ELIsA HEV IgG was induced after the injection of pcE2 or pcE3 or both,and the percentage of seraconversion was i00% after two weeks of the third iniection.Compared with injection of either construct,the antibo dy titers were higher in the group with combined injection of two constructs.

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    DNA Immunization of Hepatitis E Virus(HEV)

    • 1. Institute of Medicai Biology,CAMS/PUMC,Kunming 6501 18.China

    Abstract: Two plasmid constructs,pcE2 and peE3,containing 3’fragment of open reading frame 2 (ORF2,1163 bp)of hepatitis E virus(HEV)and full—length ORF3(369 bp).were injected into bi— lateral tibiaLs of Swiss mice respectively.for three times(0,2 and 4 weeks)and observed the HEv IgG by ELIsA HEV IgG was induced after the injection of pcE2 or pcE3 or both,and the percentage of seraconversion was i00% after two weeks of the third iniection.Compared with injection of either construct,the antibo dy titers were higher in the group with combined injection of two constructs.

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