DNA Immunization of Hepatitis E Virus(HEV)
Abstract: Two plasmid constructs,pcE2 and peE3,containing 3’fragment of open reading frame 2 (ORF2,1163 bp)of hepatitis E virus(HEV)and full—length ORF3(369 bp).were injected into bi— lateral tibiaLs of Swiss mice respectively.for three times(0,2 and 4 weeks)and observed the HEv IgG by ELIsA HEV IgG was induced after the injection of pcE2 or pcE3 or both,and the percentage of seraconversion was i00% after two weeks of the third iniection.Compared with injection of either construct,the antibo dy titers were higher in the group with combined injection of two constructs.