Construction of Gene Library and Localization of Chitinase Gene of Parocneria orienta Nuclear polyhedrovirus
Abstract: The gene library of Parocneria oriema nuclear polyhedlovims(PaorNPV)was panly constructed by doing$oiriefragnen~ ofBamH I and EcoR I ofP~rNPV genomic DNA into pGEM3Z(f)as a vector.Using the chifinase gene of Hear SNPV probed wlth[a-32p]dCTP,the chifinase gene ofPaorNPV localized OI3_the fragraents of BamH I-D,EcoR[-A,‰ dⅢ一A,Pst LD and Xho tG/H respectively.Atthe time,the fragment ofBamH I-D eontniningthe chiA has been cloned.