Citation: CU Wen-jie.LIN Jing-xiang, WANC You-chun“, ZHANC.I~a-yuan, LAN Hai.yun, LI He-rai, . The Sequence Analysts of I-IB~ S Gene Isolated from Both m 3sAgand Anti-l-IBs Antibody Positive Blood Donors .VIROLOGICA SINICA, 2001, 16(4) : 321-324.

The Sequence Analysts of I-IB~ S Gene Isolated from Both m 3sAgand Anti-l-IBs Antibody Positive Blood Donors

  • Available online: 05 December 2001
  • In order to investigate HBV genotype and variants from the blood donors positive for both HbsAg and anti HBs antibody,389 sera from HBsAg positive blood donors were tested for anti HBs antibody with RIA assay.The sera positive for both HbsAg and anti HBs were tested for HBV DNA with polymerase chain reaction(PCR).The positive PCR products were cloned and sequenced.The sequences were compared with the representative sequences of different HBV genotypes.The results showed that 10/389 sera positive for HBsAg were positive for anti HBs.Five of these ten sera were positive for HBV DNA.The sequence analysis showed that all five sequences belonged to HBV genotype B,four sequences were adw serotype and one was adr serotype.Amino acids of antigen determinant "a" of two sequences have been changed.

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    The Sequence Analysts of I-IB~ S Gene Isolated from Both m 3sAgand Anti-l-IBs Antibody Positive Blood Donors

    • 1. National Institute for the Control of Parmeceutical and Biological Products,Beijing 100050,China

    Abstract: In order to investigate HBV genotype and variants from the blood donors positive for both HbsAg and anti HBs antibody,389 sera from HBsAg positive blood donors were tested for anti HBs antibody with RIA assay.The sera positive for both HbsAg and anti HBs were tested for HBV DNA with polymerase chain reaction(PCR).The positive PCR products were cloned and sequenced.The sequences were compared with the representative sequences of different HBV genotypes.The results showed that 10/389 sera positive for HBsAg were positive for anti HBs.Five of these ten sera were positive for HBV DNA.The sequence analysis showed that all five sequences belonged to HBV genotype B,four sequences were adw serotype and one was adr serotype.Amino acids of antigen determinant "a" of two sequences have been changed.

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