Primary Studies on DNA Imm unization of Hum an Rotavirus

Abstract：The probability of DNA vaccine against human rotavirus was studied，by constructing three recombinant plasmid clones，pCI／vp7，pCI／vp4，and pCI／vp6，and transferring into TA muscle (quadriceps)of BALW C mous~．with intramuscular inoculation．The sera were collected and antibody activity was evaluated with E1 ISA and neutralizing test pCI／vp7，pCI／vp4，and pCI／vp6 have been successfully Constructed，moreover，pCl／vp7 has transfected 293 cells and the vp7 gene could expres thoroughly．Intramuscular inoculation of these plasmids induced efficient immuniation，and the anti— body induced by pCl／vp7 presents some definite protective of a strain of rotavirus Wa．As the results showed，DNA vaccine wil1 be a helpful means to protect children from severe acute diarrhea caused by Gro upA ro taviruse s．