Sequence Comparison and Analysis of L Gene from A Foot—and·month Disease Virus Strain China／99
Abstract: Foot and mouth disease virus strain China／99 RNA5 were used as templet for RT—PCR．The amplifled eDNA products were cloned into pGEM—T Easy Vectors and transformed into JM109．The re— combinant plasroids were identified by electrophoresis．E∞R l cleavage，and PCR The nucleotide and amino acid sequences were compared with the I，genes of the other four reference strains．The resuIts shown that the differential ratio of L gene of China／99 with A10．OIK，OlCaropos and TW45 strains was l5 27％ ，15．56％ ．15 56％ and 15 49％ respectively；and that of amio acid se0uences was 8．29％．8．76％。9 22％ and 10 14％ respectively．All the conjunctions of L／el for five fot—and— mouth viruses are Gly／lie．The higher conversions in T．C．A-G and A-c mostly affect the stabgle of amino acid residuses． The regions of 43．53th． 95．105th， 108—11lth， 146—153th 。161—173th． 183— 188th and 182·187[h in I protease roost probably are the active sites that play an important role 1n keeping configuration andfunction of proteins，andH 8．C5l，E65，H95．H109，Hl38，Hl48 andEI65may be active amino acids
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