Citation: SUN Xue-qiang, LU Cheng-ping. Isolation and Purification of the Eriocheir sinensis “Tremble Disease”Virus and Its Pathongenity on Crabs .VIROLOGICA SINICA, 2002, 17(2) : 185-188.

Isolation and Purification of the Eriocheir sinensis “Tremble Disease”Virus and Its Pathongenity on Crabs

  • Available online: 05 April 2002
  • Abstract:The Eriocheir sinensis(Es)that suffered“tremble diease”by artificial infection were ho— mogenated The supernatant was concentrated with p。】yethyIeneg1ycol(PEG)6000 and purified by gel filtration chromatogmph with Sepia,arose 4B.The eluates collected were concentrated at 4℃ .The virus particles in the first part of the eluates could be seen under electron microscopy.The diameter of virions varified jn 50~ 100nm Sinopotamon denticu缸£“?II artilicia】inlected with the purified virus became j1].Bv double antibody sandwich EI ISA detection.the results showed that there was signifi— cai]t diflerence between the jnfected and the centre crabs

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    Isolation and Purification of the Eriocheir sinensis “Tremble Disease”Virus and Its Pathongenity on Crabs

    • 1. Kay lab of Aniatat Disease Diagnosis& tmmunotogy,Ministry of Agriculture,Nanjing Agri Univ,Nanjing 210095,China

    Abstract: Abstract:The Eriocheir sinensis(Es)that suffered“tremble diease”by artificial infection were ho— mogenated The supernatant was concentrated with p。】yethyIeneg1ycol(PEG)6000 and purified by gel filtration chromatogmph with Sepia,arose 4B.The eluates collected were concentrated at 4℃ .The virus particles in the first part of the eluates could be seen under electron microscopy.The diameter of virions varified jn 50~ 100nm Sinopotamon denticu缸£“?II artilicia】inlected with the purified virus became j1].Bv double antibody sandwich EI ISA detection.the results showed that there was signifi— cai]t diflerence between the jnfected and the centre crabs

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